HPLC Analysis of Peptides on Discovery^® BIO Wide Pore C18 (Gradient #2)

column

Discovery BIO Wide Pore C18, 15 cm × 4.6 mm I.D., 5 μm particles (568222-U)

mobile phase

[A] (80:20), 0.1%TFA in water:0.1% TFA in acetonitrile; [B] (66:34), 0.1% TFA in water:0.1% TFA in acetonitrile

flow rate

1 mL/min

column temp.

30 °C

detector

UV, 215 nm

injection

5 μL Sigma Peptide Mix P2693 (~25 μg ea. Peptide) (~25 μg ea. peptide)

sample

Sigma Peptide Mix (Cat. No. P2693)