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Merck

HPLC Analysis of Plasmids on TSKgel® DNA-NPR

HPLC Analysis of Plasmids on TSKgel® DNA-NPR application for HPLC

材料

分析柱

產品號碼
描述
訂價

818249

TSKgel® DNA-NPR HPLC Column

phase DEAE (diethylaminoethyl), L × I.D. 7.5 cm × 4.6 mm, 2.5 μm particle size

流動相成分

產品號碼
描述
訂價

S3014

氯化钠

for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)

10812846001

三(羟甲基)氨基甲烷盐酸盐

>99% (titration), pH 7.0-9.0, suitable for FISH

防護柱

產品號碼
描述
訂價

818253

TSK-GEL® 阴离子交换 HPLC 保护柱

phase DEAE (diethylaminoethyl), L × I.D. 0.5 cm × 4.6 mm, 2.5 μm particle size

CONDITIONS

column

TSKgel DNA-NPR, 7.5 cm x 4.6 mm, 2.5 μm particles (818249)

mobile phase

[A] 0.02 M Tris, pH 9.0; [B] 0.02 M Tris, 1.0 M NaCl, pH 9.0

gradient

50 to 60% B in 10 column volumes

flow rate

1 mL/min

detector

UV, 260 nm

sample

PUC19 plasmid

描述

其他說明

Data provided by Tosoh Bioscience LLC.

法律資訊

TSKgel is a registered trademark of Tosoh Corporation

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HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS