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Merck

HPLC Analysis of Caffeine and Taurine on Ascentis® Express HILIC Column using Evaporative Light Scattering Detection (ELSD, Fast Analysis)

HPLC Analysis of Caffeine and Taurine on Ascentis® Express HILIC Column using Evaporative Light Scattering Detection (ELSD, Fast Analysis) application for HPLC

材料

分析柱

產品號碼
描述
訂價

53967-U

Ascentis® Express HILIC, 2.7 μm HPLC色谱柱

2.7 μm particle size, L × I.D. 5 cm × 3 mm

標準

產品號碼
描述
訂價

T0625

牛磺酸

≥99%

56396

咖啡因

certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

CONDITIONS

column

Ascentis Express HILIC, 5 cm x 3.0 mm I.D., 2.7 μm particles (53967-U)

mobile phase

[A] 100 mM ammoniumformate, pH 3.0 with formic acid; [B] acetonitrile; (15:85, A:B)

flow rate

0.6 mL/min

column temp.

25 °C

detector

ELSD, 55 °C, 3.5 bar nitrogen

injection

2 μL

sample

1000 μg/mL in 10:90, water: acetonitrile

描述

分析報告

This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of caffeine and taurine. Elution is by aqueous normal phase (ANP) as an increase in organic will increase retention.

法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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