GC Analysis of a 20-Component Organochlorine Pesticide Mix in Water on a 5% Phenyl Column after SPE using Supelclean^™ ENVI^™-8

sample preparation

SPE (Solid Phase Extraction)

sample/matrix

waste water spiked with pesticides

SPE tube/cartridge

Supelclean ENVI-8, glass hardware, 500 mg/6 mL (57107 (500 mg/6 mL); 57108 (1 g/6 mL))

condition

3 mL methanol; h 2 mL 5% methanol in water

sample addition

load sample at 5 mL/min

drying

2-3 min with nitrogen stream

elution

2 x 4 mL hexane:acetone (90:10), drop wise. allow to soak into bed

column

5% Phenyl Polysiloxane, 15 m × 0.53 mm I.D., 0.50 μm (Supelco equivalent, Equity-5, 28252-U)

oven

150 °C (0.5 min) to 275 °C (5 min)

inj. temp.

200 °C

detector

ECD, 300 °C

carrier gas

helium

injection

1 μL split/splitless (45 sec delay)