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Merck
  • Hollow microneedle-mediated intradermal delivery of model vaccine antigen-loaded PLGA nanoparticles elicits protective T cell-mediated immunity to an intracellular bacterium.

Hollow microneedle-mediated intradermal delivery of model vaccine antigen-loaded PLGA nanoparticles elicits protective T cell-mediated immunity to an intracellular bacterium.

Journal of controlled release : official journal of the Controlled Release Society (2017-09-18)
Anne Marit de Groot, Guangsheng Du, Juha Mönkäre, Anouk C M Platteel, Femke Broere, Joke A Bouwstra, Alice J A M Sijts
摘要

The skin is an attractive organ for immunization due to the presence of a large number of epidermal and dermal antigen-presenting cells. Hollow microneedles allow for precise and non-invasive intradermal delivery of vaccines. In this study, ovalbumin (OVA)-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles with and without TLR3 agonist poly(I:C) were prepared and administered intradermally by hollow microneedles. The capacity of the PLGA nanoparticles to induce a cytotoxic T cell response, contributing to protection against intracellular pathogens, was examined. We show that a single injection of OVA-loaded PLGA nanoparticles, compared to soluble OVA, primed both adoptively transferred antigen-specific naïve transgenic CD8

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Sigma-Aldrich
3,6-二甲基-1,4-二氧杂环己烷-2,5-二酮, 99%
Sigma-Aldrich
乙交酯, ≥99%