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  • PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice.

PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice.

Nature communications (2017-10-12)
Deqiang Ding, Jiali Liu, Kunzhe Dong, Uros Midic, Rex A Hess, Huirong Xie, Elena Y Demireva, Chen Chen
摘要

Piwi-interacting RNAs are small regulatory RNAs with key roles in transposon silencing and regulation of gametogenesis. The production of mature piwi-interacting RNAs requires a critical step of trimming piwi-interacting RNA intermediates to achieve optimally sized piwi-interacting RNAs. The poly(A)-specific ribonuclease family deadenylase PNLDC1 is implicated in piwi-interacting RNA trimming in silkworms. The physiological function of PNLDC1 in mammals remains unknown. Using Pnldc1-deficient mice, here we show that PNLDC1 is required for piwi-interacting RNA biogenesis, transposon silencing, and spermatogenesis. Pnldc1 mutation in mice inhibits piwi-interacting RNA trimming and causes accumulation of untrimmed piwi-interacting RNA intermediates with 3' end extension, leading to severe reduction of mature piwi-interacting RNAs in the testis. Pnldc1 mutant mice exhibit disrupted LINE1 retrotransposon silencing and defect in spermiogenesis. Together, these results define PNLDC1 as a mammalian piwi-interacting RNA biogenesis factor that protects the germline genome and ensures normal sperm production in mice.piRNAs are regulatory RNAs that play a critical role in transposon silencing and gametogenesis. Here, the authors provide evidence that mammalian PNLDC1 is a regulator of piRNA biogenesis, transposon silencing and spermatogenesis, protecting the germline genome in mice.

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抗-β-肌动蛋白−过氧化物酶抗体,小鼠单克隆 小鼠抗, clone AC-15, purified from hybridoma cell culture
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抗磷酸组蛋白H2A.X(Ser139)抗体,克隆JBW301,FITC结合物, clone JBW301, Upstate®, from mouse