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  • [Reaction of unconjugated and conjugated bilirubin with Fast Red TR and related diazonium salts].

[Reaction of unconjugated and conjugated bilirubin with Fast Red TR and related diazonium salts].

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie (1985-11-01)
G E Hoffmann, R Hiefinger, L Weiss
摘要

In the present study, the reaction of 15 different diazonium salts with serum bilirubin is examined. It is shown that the selectivity of the determination of non-conjugated and total bilirubin greatly depends on two conditions: the pH-value of the reaction mixture and the nature and position of substituents within the benzene ring. Introduction of "electron-pushing" groups such as the o-methyl group of Fast Red TR will change the reactivity of the diazonium cation towards bilirubin in such a way that the co-reaction of the conjugated fraction becomes negligible at pH-values between 2 and 5. After neutralization to pH 7 the total bilirubin can be determined.

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Sigma-Aldrich
固红-锌TR盐, Dye content 15 %
Sigma-Aldrich
Fast Red TR盐 半(氯化锌) 盐