ZRB1673

Anti-p-IKKα/β-Ser176/180 Antibody, clone 2G2 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 分類程式碼代碼: 12352203
• NACRES: NA.43

屬性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 2G2, recombinant monoclonal
• 描述: recombinant, expressed in HEK 293 cells
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 84.64 kDa; observed mol wt ~45 kDa
• 純化經由: using Protein A
• 物種活性: human
• 物種活性（以同源性預測）: canine, bovine, mouse, porcine, rat, monkey, feline
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: affinity binding assay: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; inhibition assay: suitable; western blot: suitable
• 同型: IgG
• 表位序列: N-terminal half
• Protein ID登錄號: NP_001269
• UniProt登錄號: O15111
• 環保替代類別: , Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: phosphorylation (pSer176/pSer180)
• 基因資訊: human ... CHUK(1147)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 2G2 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects IKKa/β phosphorylated on serine 176 and 180.

免疫原

KLH-conjugated linear peptide corresponding to 13 amino acids surrounding phosphoserine 176 and 180 from human IKKa.

應用

Quality Control Testing

Evaluated by Western Blotting in a construct containing GST-tagged recombinant IKK /β protein fragment phosphorylated on Ser176/180.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected a construct containing GST-tagged recombinant IKK /β protein fragment phosphorylated on Ser176/180, but did not detect in non-phosphorylated construct. (Phospho- IKK /β construct: Courtesy of Dr. Jesse Rinehart, Yale University, School of Medicine).

Tested applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected phospho-IKK /β-Ser176/180 in human colon cancer tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound Phospho-IKK /β- Ser176/180 peptide with a KD of 3.2 x 10-7 in an affinity binding assay.

Peptide Inhibition Assay: Target band detection in a construct containing GST-tagged recombinant IKKa protein fragment phosphorylated on Ser 176/180 was prevented by pre-blocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

標靶描述

Inhibitor of nuclear factor kappa-B kinase subunit alpha (UniProt: O15111; also known as EC:2.7.11.10, I-kappa-B kinase alpha, IKK-A, IKK-alpha, IkBKA, IkappaB kinase, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase 1, IKK1, Nuclear factor NF-kB inhibitor kinase alpha, NFKBIKA, Transcription factor 16, TCF-16) is encoded by the CHUK (also known as IKKA, TCF16) gene (Gene ID:1147) in human. The activation of NF-kB by extracellular inducers depends on the phosphorylation and subsequent degradation of IkkB proteins. Activation of NF-kB is achieved through the action of a family of kB kinases (IKK). IKK contain two catalytic subunits (IKKa and IKKβ) and a regulatory/adapter protein IKK (NEMO). IKK and IKKβ play an essential role in NF-kB signal transduction that is activated by multiple stimuli. Their protein kinase domains are localized in amino acids 15-302 (IKK ) and 15-300 (IKKβ). Their leucine-zipper, localized in amino acids 455-476 (for IKKa) and 458-479 (for IKKβ), is important to allow for their homo- and hetero-dimerization. IKKa/IKKβ act as part of the canonical IKK complex in the conventional pathway of NF-kB activation and phosphorylate serine residues on IkBa and IkBβ. Although IKK and IKKβ share about 50% sequence homology, they can interchangeably phosphorylate Ser32/36 of IkBa and Ser 19/23 of IkBβ. These phosphorylation events lead to immediate polyubiquitination and degradation of IkB proteins and allow NF-kB to translocate to the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, and protection against apoptosis. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Solt, LA., and May, MJ. (2008). Immunol. Res. 42(1-3); 3-18; Regnier, CH., et al. (1997). Cell. 90(2); 373-383).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 13 - Non Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable