推薦產品
生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
3M6, recombinant monoclonal
描述
recombinant, expressed in HEK 293 cells
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 36.11 kDa
observed mol wt ~36 kDa
純化經由
using Protein A
物種活性
human
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable
同型
IgG
表位序列
Unknown
Protein ID登錄號
UniProt登錄號
環保替代類別
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... EIF2S1(1965)
相關類別
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特異性
Clone 3M6 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Eukaryotic translation initiation factor 2a (eIF-2a).
免疫原
His-tagged recombinant full-length human Eukaryotic translation initiation factor 2 subunit a (eIF-2a )
應用
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected eIF-2a in HeLa cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected eIF-2a in Raji cell lysate.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected eIF-2a in HeLa cell line, A431, HUVEC, and NIH 3T3 cells.
Affinity Binding Assay:: A representative lot of this antibody bound eIF-2a with a KD of 2.6 x 10-8 in an affinity binding assay.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected eIF-2a in one million Ramos cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected eIF-2a in HeLa cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected eIF-2a in Raji cell lysate.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected eIF-2a in HeLa cell line, A431, HUVEC, and NIH 3T3 cells.
Affinity Binding Assay:: A representative lot of this antibody bound eIF-2a with a KD of 2.6 x 10-8 in an affinity binding assay.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected eIF-2a in one million Ramos cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
標靶描述
Eukaryotic translation initiation factor 2 subunit 1 (UniProt: P05198; also known as Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha) is encoded by the EIF2S1 (also known as EIF2A) gene (Gene ID: 1965) in human. eIF-2A is a heterotrimer composed of a, b, and g chains. It functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. Its activity is regulated by phosphorylation at serine 48 and serine 51 (without methionine), which stabilizes the eIF-2/GDP/eIF-2B complex and prevents the GDP/GTP exchange reaction, thus impairing the recycling of eIF-2a between successive rounds of initiation and leading to global inhibition of translation. elF-2a is reported to be a substrate for eukaryotic translation initiation factor 2-alpha kinase 1 to 4 (EIF2AK1, 2, 3, and 4). It is also a key component of the integrated stress response (ISR) and is required for adaptation to various stress conditions. Phosphorylation on serine 51 by the EIF2AK4 is shown to occur in response to amino acid starvation and UV irradiation. Its phosphorylation by EIF2AK1-4 converts it into a global protein synthesis inhibitor, leading to reduction in cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Lee, YY., et al. (2009). J. Biol. Chem. 284(11); 6661-6673; Mikami, S., et al. (2006). Protein Expr, Purif. 46(2); 348-357).
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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