SRP0103

HDAC2 FLAG tag human

recombinant, expressed in baculovirus infected insect cells, ≥50% (SDS-PAGE)

• 同義詞: HD2, Histone deacetylase 2, Transcriptional regulator homolog RPD3, YAF1, YY1-associated factor 1
• 分類程式碼代碼: 12352200
• NACRES: NA.77

屬性

• 生物源: human
• 重組細胞: expressed in baculovirus infected insect cells
• 化驗: ≥50% (SDS-PAGE)
• 形狀: aqueous solution
• 分子量: 60 kDa
• 包裝: pkg of 50 μg
• 儲存條件: avoid repeated freeze/thaw cycles
• 濃度: >0.02 mg/mL
• NCBI登錄號: Q92769
• UniProt登錄號: Q92769
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... HDAC2(3066)

描述

一般說明

Human HDAC2, GenBank Accession No. Q92769, full length with C-terminal DDDDK tag (FLAG), MW = 60 kDa, expressed in baculovirus expression system.
The HDAC2 (histone deacetylase 2) gene is mapped to human chromosome 6q21 and is ubiquitously expressed.

應用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

生化／生理作用

Histone deacetylase 2 (HDAC2) is known to regulate DNA damage signalling, maintain genomic stability and prevents tumor formation and progression. Hdac2 does not possess a DNA binding domain, it forms multi-component repressor complexes for its interaction with transcription factors. Hdac2 also mediates cell proliferation, differentiation and apoptosis. HDAC2 controls response to chronic inflammation in COPD (chronic obstructive pulmonary disease). It activates Wnt signaling pathway via β-catenin T-cell transcription factor. Deletion of HDAC2 along with HDAC1 leads to anemia and thrombocytopenia.

單位定義

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

外觀

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20 and 10% glycerol.

準備報告

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.