S9915

干细胞因子 来源于小鼠

SCF, recombinant, expressed in E. coli, powder, suitable for cell culture

• 同義詞: SCF, c-Kit ligand
• MDL號碼: MFCD00132378
• 分類程式碼代碼: 12352202
• NACRES: NA.77

屬性

• 生物源: mouse
• 品質等級: 200
• 重組細胞: expressed in E. coli
• 化驗: ≥95% (SDS-PAGE and HPLC)
• 形狀: powder
• 效力: ≤10 ng/mL ED₅₀/EC₅₀
• 品質: endotoxin tested
• 分子量: 18.3 kDa
• 包裝: pkg of 10 μg; pkg of 50 μg
• 儲存條件: avoid repeated freeze/thaw cycles
• 技術: cell culture | mammalian: suitable
• 雜質: <1 EU/μg
• 顏色: white
• 溶解度: water: soluble
• UniProt登錄號: P20826
• 儲存溫度: −20°C
• 基因資訊: mouse ... Kitl(17311) , kitl(17311)

描述

一般說明

Stem cell factor (SCF), also known as the c-kit ligand, is produced by the gastrointestinal smooth muscle cells, enteric neurons, endothelial cell, lung fibroblast and bone marrow adipocytes. The SCF gene is mapped to murine chromosome 10.
Recombinant mouse stem cell factor (SCF) is a 18.3 kDa protein containing 164 amino acid residues.

應用

SCF, also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.

Stem Cell Factor from mouse has been used in the evaluation of in vitro 5-lipoxygenase inhibition by measuring the formation of LTC4.

生化／生理作用

Stem cell factor is responsible for cellular development, proliferation, migration and survival, specially observed with cardiac stem cells. This phenomenon is mediated by stem cell factor (SCF) through chemotaxis. SCF is involved in repair mechanism of myocardial infarctions. SCF signalling is responsible for the function and survival rate of interstitial cells of Cajal. Decreased level of SCF leads to gastrointestinal motility disorders. SCF might be associated with systemic vascular remodeling. SCF signalling is also believed to contribute to the pathogenesis of pulmonary hypertension.

Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells.

外觀

Lyophilized from a 0.2 μm filtered buffered solution.

分析報告

The biological activity is measured by the dose-dependent stimulation of the proliferation of human TF-1 cells.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, type N95 (US)