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一般說明
Neuroporter™ 转染试剂是经过优化的专利阳离子脂质体配方,毒性低,可用于向原代神经细胞、神经胶质细胞以及培养的神经细胞系之中高效递送DNA。Neuroporter™转染试剂盒可用于难转染原代神经细胞,可解决诸如细胞活力不足、转染效率低以及神经细胞退化等之前存在的问题。
應用
适于向原代神经细胞和培养的神经细胞系之中瞬时和稳定转染核酸。每一块6 cm细胞培养板可使用大约15-120 μl Neuroporter™的转染试剂和6-8 μg 的DNA(可应客户要求,以溶于独特的DNA稀释液的形式提供)。以下细胞已采用Neuroporter™转染试剂盒成功转染。
- C6胶质瘤细胞(人类)
- 皮质神经元细胞(大鼠原代细胞)
- 背根神经节(DRG)细胞(大鼠)
- NT2神经细胞(人前体细胞)
- NT神经元(人分化细胞)
- 室下区 (SVZ)细胞(小鼠)
- 白质细胞(小鼠)
特點和優勢
- 针对原代神经细胞、神经胶质细胞以及培养的神经细胞系进行了优化
- 具有超低的细胞毒性,不会造成神经细胞退化或树突戒断
- 可有效地递送原代神经细胞、神经胶质细胞以及培养的神经细胞系
- 相对于其他方法,使用更为轻松、快捷
- 兼容含血清和不含血清的转染实验
成分
1小瓶Neuroporter™ 转染试剂,为干燥的脂质体膜形式(T2823)
1.5 mL缓冲液 H9036
7.5 mL DNA稀释液D1941
1.5 mL缓冲液 H9036
7.5 mL DNA稀释液D1941
注意
注意:不要冻存。
原則
在无血清的条件下混合Neuroporter™转染试剂和DNA,即可形成稳定的非共价偶联复合物。该复合物性质稳定,可直接加入到细胞培养基中,并在培养基中与细胞膜融合,将DNA释放到细胞质中。注意:复合物的形成受到血清抑制,但一旦形成稳定的复合物,即便存在血清也无妨。
法律資訊
NeuroPorter is a trademark of Gene Therapy Systems, Inc.
儲存類別代碼
12 - Non Combustible Liquids
閃點(°F)
Not applicable
閃點(°C)
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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瀏覽我們便捷的轉染試劑選擇指南,為您的特定細胞系和應用需求匹配最佳試劑。
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