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KCQS03

Sigma-Aldrich

KiCqStart® SYBR® Green qPCR ReadyMix预混液

iQ, with fluorescein for Bio-Rad systems

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About This Item

分類程式碼代碼:
41106300
NACRES:
NA.55

形狀

liquid

用途

sufficient for 1250 reactions
sufficient for 250 reactions
sufficient for 5000 reactions

特點

dNTPs included
hotstart

儲存條件

protect from light

技術

qPCR: suitable

顏色

colorless

輸入

purified DNA

相容性

for use with Bio-Rad MyiQ
for use with Bio-Rad iCycler iQ
for use with Bio-Rad iQ 5

檢測方法

SYBR® Green

運輸包裝

dry ice

儲存溫度

−20°C

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一般說明

KiCqStart SYBR Green qPCR ReadyMix是2倍浓缩的即用型预混液,含有除引物和模板之外的实时定量PCR(qPCR)所需的全部组分。这种专有缓冲液、稳定剂和Taq DNA热启动聚合酶的独特组合使用快速或常规的SYBR Green qPCR循环方案,具有最高的PCR效率、灵敏度、特异性和稳定可靠的荧光信号。

高特异性扩增对于使用SYBR Green I染料技术成功进行qPCR至关重要,因为该染料可结合扩增过程中产生的任何dsDNA并进行检测。Taq DNA热启动聚合酶在PCR变性步骤开始之前,处于抗体介导的失活状态。

應用

PCR扩增:
  • 基因表达
  • DNA定量
  • 染色质免疫沉淀(CHiP)
KiCqStart® SYBR® Green qPCR ReadyMix has been used for the qPCR analysis of 16S rRNA.
Different real-time PCR systems employ different strategies for the normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument. KiCqStart SYBR Green qPCR ReadyMix, iQ contains fluorescein for experimental plate well factor collection on iCycler iQ real-time detection systems or the MyiQ detection system.

特點和優勢

  • 只需33分钟即可获得分析结果
  • 高效、灵敏的实时PCR结果
  • 几乎不需要多少优化,甚至无需优化

成分

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), KiCqStart Taq DNA Polymerase, SYBR Green dye, 20 nM fluorescein, and stabilizers

packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

其他說明

储存条件:
KiCqStart SYBR Green qPCR ReadyMix在-20°C的恒温冷冻柜中避光保存,可稳定存放1年。为方便使用,也可在+2至+8°C下储存长达6周。解冻后,充分混匀后再使用。不建议反复冻融产品。不过,冻融20次或+20°C存放2个月后,产品没有出现任何性能损失。

法律資訊

KiCqStart is a registered trademark of QIAGEN Beverly Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
iQ is a trademark of Bio-Rad Laboratories, Inc.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Jackson T Sparks et al.
Insect biochemistry and molecular biology, 43(12), 1161-1171 (2013-10-26)
The yellow-fever mosquito, Aedes aegypti, infects a growing number of people every year with dengue, yellow fever and chikungunya viruses. Contact chemoreception in mosquitoes influences a number of behaviors including host-selection, oviposition and feeding. While these behaviors are in many
Himanshu Kumar Khuntia et al.
SN applied sciences, 2(8), 1320-1320 (2020-08-25)
This research aims to determine the presence of antibiotic-resistant genes (ARG) in anaerobic biofilm reactors (ABR) fed with household chemical products (HCP) such as laundry detergents and handwash without any influx of antibiotics. The ABR comprised a three-chamber design with
Glade Dlott et al.
Journal of microbiological methods, 115, 112-120 (2015-06-10)
We tested a method of estimating the activity of detectable individual bacterial and archaeal OTUs within a community by calculating ratios of absolute 16S rRNA to rDNA copy numbers. We investigated phylogenetically coherent patterns of activity among soil prokaryotes in
Andreas Varkaris et al.
International journal of cancer, 133(7), 1536-1546 (2013-03-26)
The receptor tyrosine kinase, MET, has been implicated in tumorigenesis and metastasis of many solid tumors, by multiple mechanisms, including cross talk with epidermal growth factor receptor. In this study, we examined the role of insulin-like growth factor receptor-1 (IGF-1R)
Jonathan D Bohbot et al.
Insect biochemistry and molecular biology, 48, 29-39 (2014-03-13)
Female yellow-fever mosquitoes, Aedes aegypti, are obligate blood-feeders and vectors of the pathogens that cause dengue fever, yellow fever and Chikungunya. This feeding behavior concludes a series of multisensory events guiding the mosquito to its host from a distance. The

文章

After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.

傳統 PCR 完成後,PCR/qPCR 資料分析是透過瓊脂質凝膠或最近的毛細管進行解析。

PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.

PCR 分析指南引導您進行引物驗證和其他分析最佳化因素,以確保高靈敏度和特異性,達到最佳的 DNA/ RNA 定量。

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條款

使用SYBR Green试剂的定量PCR实验方案。该方法支持绝大多数qPCR仪器。

Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.

基因表达数据的分析需要稳定的参考或上样控制。所述参考通常是一个或多个参照基因。

Analysis of gene expression data requires a stable reference or loading control. This reference is usually one or more reference genes.

相關內容

SYBR® Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. Explore our LuminoCt® and KiCqStart® products for Fast qPCR or JumpStart™ reagents for conventional qPCR

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

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