GE28-4082-61

HiTrap^® MabSelect^™ Xtra

Cytiva 28-4082-61, pack of 5 × 5 mL

• 分類程式碼代碼: 23151816
• NACRES: NA.56

屬性

• ligand: recombinant protein A (E. coli)
• 包裝: pack of 5 × 5 mL
• 製造商／商標名: Cytiva 28-4082-61
• 儲存條件: (20% Ehtanol); (20% Ethanol)
• 參數: 42 psi
• 床尺寸: 16 mm × 25 mm
• 床體積: 5 mL
• 柱內徑: 16 mm
• 基質: highly cross-linked agarose
• 樣品平均尺寸: 75 (d₅₀v is the median particle size of the cumulative volume distribution)
• cleaning: 2-12
• 工作範圍: 3-10
• 容量: ≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)
• 適合性: suitable for bioprocess medium
• 儲存溫度: 2-8°C

描述

一般說明

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.

HiTrap^® MabSelect^™ Xtra columns are prepacked with MabSelect^™ Xtra, a BioProcess medium for capture of monoclonal antbodies from large sample volumes.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

應用

The increased capacity of the medium is well suited for purification of Mabs from high-level expression feedstock.

特點和優勢

• Increased dynamic binding capacity with smaller particle size and greater porosity than MabSelect^™ medium.
• MabSelect^™ Xtra uses the same recombinant Protein A ligand as MabSelect^™
• Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
• Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
• Prepacked HiTrap^® columns for process development, screening of purification conditions, and small-scale purification of Mabs.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他說明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律資訊

HiTrap is a registered trademark of Cytiva

MabSelect is a trademark of Cytiva

安全資訊

• 象形圖: GHS02
• 訊號詞: Warning
• 危險聲明: H226
• 防範說明: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 儲存類別代碼: 3 - Flammable liquids