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應用
可用于通过 O′Brien, W., Anal. Biochem., FB76, 423 (1976) 介绍的方法进行焦磷酸盐测定。
生化/生理作用
Fructose-1,6-bisphosphatase (FBP) is an important enzyme in glucose metabolism. It catalyzes the hydrolysis of fructose-1,6-bisphosphate to fructose-6-phosphate and inorganic phosphate.[1] Fructose-6-phosphate kinase converts fructose-6-phosphate into fructose 1,6-bisphophate in the rate limiting step of the glycolysis cycle.[2][3]
其他說明
这两种焦磷酸依赖性果糖-6-磷酸激酶的区别在于它们对生理活化剂果糖 2,6-二磷酸 [Fru (2,6)-P2]的反应不同。据报道,来自费氏丙酸杆菌的酶 (F8381) 不受 Fru (2,6)-P2 影响。而来自马铃薯块茎的酶 (F2258) 则可被 Fru (2,6)-P2 激活(在 5.5nM Fru (2,6)-P2 条件下,半最大活化马铃薯块茎酶)。
單位定義
在 pH 7.4 和 30°C 下,一单位每分钟可将 1.0μmol 的焦磷酸盐和果糖-6-磷酸转化为果糖-1,6-二磷酸和无机磷酸盐。
外觀
含咪唑盐和稳定剂
訊號詞
Danger
危險聲明
危險分類
Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1C
儲存類別代碼
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Inorganic pyrophosphate dependent D-fructose-6-phosphate 1-phosphotransferase from Propionibacterium freudenreichii was purified to apparent homogeneity by the criterion of silver staining on sodium dodecyl sulfate (SDS) gels. In the direction of phosphorylation of fructose 6-phosphate (F6P), an intersecting initial velocity pattern is
Corrigendum: Online Pyrophosphate Assay for Analyzing Adenylation Domains of Nonribosomal Peptide Synthetases.
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A major problem in defining the physiological role of pyrophosphate:fructose 6-phosphate 1-phosphotransferase (PFP, EC 2.7.1.90) is the 1,000-fold discrepancy between the apparent affinity of PFP for its activator, fructose 2,6-bisphosphate (Fru-2,6-P2), determined under optimum conditions in vitro and the estimated
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Pyrophosphate-dependent phosphofructokinase (PPi-PFK) has been detected in several types of plant cells, but the gene has not been reported in sugar cane. Using Citrus paradisi PPi-PFK gene (AF095520 and AF095521) sequences to search the sugar cane EST database, we have
相關內容
Enzymatic Assay of Fructose-6-Phosphate Kinase Pyrophosphate Dependent
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