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重要文件

F8381

Sigma-Aldrich

焦磷酸依赖性果糖-6-磷酸激酶 来源于费氏丙酸杆菌(谢氏亚种)

lyophilized powder, ≥4.0 units/mg protein

同義詞:

6-磷酸果糖激酶(焦磷酸盐), 焦磷酸盐: D-果糖-6-磷酸 1-磷酸转移酶

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About This Item

CAS號碼:
酶委員會編號:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54
暫時無法取得訂價和供貨情況

形狀

lyophilized powder

品質等級

比活性

≥4.0 units/mg protein

成份

Protein, 5.0-25.0% biuret

儲存溫度

−20°C

應用

可用于通过 O′Brien, W., Anal. Biochem., FB76, 423 (1976) 介绍的方法进行焦磷酸盐测定。

生化/生理作用

Fructose-1,6-bisphosphatase (FBP) is an important enzyme in glucose metabolism. It catalyzes the hydrolysis of fructose-1,6-bisphosphate to fructose-6-phosphate and inorganic phosphate.[1] Fructose-6-phosphate kinase converts fructose-6-phosphate into fructose 1,6-bisphophate in the rate limiting step of the glycolysis cycle.[2][3]

其他說明

这两种焦磷酸依赖性果糖-6-磷酸激酶的区别在于它们对生理活化剂果糖 2,6-二磷酸 [Fru (2,6)-P2]的反应不同。据报道,来自费氏丙酸杆菌的酶 (F8381) 不受 Fru (2,6)-P2 影响。而来自马铃薯块茎的酶 (F2258) 则可被 Fru (2,6)-P2 激活(在 5.5nM Fru (2,6)-P2 条件下,半最大活化马铃薯块茎酶)。

單位定義

在 pH 7.4 和 30°C 下,一单位每分钟可将 1.0μmol 的焦磷酸盐和果糖-6-磷酸转化为果糖-1,6-二磷酸和无机磷酸盐。

外觀

含咪唑盐和稳定剂

象形圖

Health hazardCorrosion

訊號詞

Danger

危險聲明

危險分類

Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1C

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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Daniel L Machell et al.
Chembiochem : a European journal of chemical biology, 23(18), e202200325-e202200325 (2022-07-26)
Detection of pyrophosphate is important in quantifying enzyme activity, particularly adenylation domain activity during non-ribosomal peptide synthesis. The previous development of an enzyme coupled PPi /NADH assay allowed the measurement of such activity in an online fashion using commercially available
B L Bertagnolli et al.
Biochemistry, 23(18), 4101-4108 (1984-08-28)
Inorganic pyrophosphate dependent D-fructose-6-phosphate 1-phosphotransferase from Propionibacterium freudenreichii was purified to apparent homogeneity by the criterion of silver staining on sodium dodecyl sulfate (SDS) gels. In the direction of phosphorylation of fructose 6-phosphate (F6P), an intersecting initial velocity pattern is
Corrigendum: Online Pyrophosphate Assay for Analyzing Adenylation Domains of Nonribosomal Peptide Synthetases.
Tiia Kittilä et al.
Chembiochem : a European journal of chemical biology, 23(18), e202200381-e202200381 (2022-07-28)
M E Theodorou et al.
Planta, 213(1), 147-157 (2001-08-29)
A major problem in defining the physiological role of pyrophosphate:fructose 6-phosphate 1-phosphotransferase (PFP, EC 2.7.1.90) is the 1,000-fold discrepancy between the apparent affinity of PFP for its activator, fructose 2,6-bisphosphate (Fru-2,6-P2), determined under optimum conditions in vitro and the estimated
J Suzuki et al.
Genetics and molecular research : GMR, 2(4), 376-382 (2004-03-11)
Pyrophosphate-dependent phosphofructokinase (PPi-PFK) has been detected in several types of plant cells, but the gene has not been reported in sugar cane. Using Citrus paradisi PPi-PFK gene (AF095520 and AF095521) sequences to search the sugar cane EST database, we have

相關內容

Enzymatic Assay of Fructose-6-Phosphate Kinase Pyrophosphate Dependent

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