IL-A50

91072524, Reactivity: Anti-IgM (polymorphic determinant), bovine

• 分類程式碼代碼: 41106514

屬性

• 生物源: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• 骨髓瘤: P3X63Ag8.653
• 反應: Anti-IgM (polymorphic determinant), bovine
• 技術: cell culture | mammalian: suitable
• 同型: IgG2a
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Mouse P3X63Ag8.653 x Mouse BALB/c spleen

細胞系描述

The antibody is directed against a polymorphic determinant on bovine IgM. It reacts with serum IgM and IgM+ B cells, precipitating polypeptide chains of 80kDa and 28kDa. It does not react with bovine IgG.

免疫原

Bovine PBMs, depleted of T lymphocytes

應用

Evaluation of bovine immunoglobulin levels and lymphocyte populations

培養基

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

例行更新培養

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. These cells may require mouse macrophage feeder cells upon resuscitation.

其他說明

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