UT-1

87100701, hamster ovary, Epithelial

• 分類程式碼代碼: 41106514

屬性

• product name: UT-1, 87100701
• 生物源: hamster ovary
• 增長模式: Adherent
• 染色體組型: 2n = 22
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Hamster Chinese ovary

細胞系描述

Derived from parent line CHO-K1. Cells were exposed to increasing concentrations of compactin over 8 months. During this exposure the level of 3-hydroxy-3-methylglutaryl coenzyme A reductase synthesis increased 500-fold. The enzyme controls cholesterol synthesis.

應用

Enzymatic studies

培養基

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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