推薦產品
生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
18-2, monoclonal
形狀
liquid
包含
≤0.1% sodium azide as preservative
物種活性
human
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
同型
IgG1
運輸包裝
wet ice
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... PRKDC(5591)
一般說明
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing the splenocytes with mouse myeloma SP2/0 cells (see application references). Recognizes the ~460 kDa DNA-PK protein.
Recognizes the ~470 kDa DNA-PK protein in A431, HeLa, and HL60 cells, normal tonsil, normal colon, and breast carcinoma tissue.
This Anti-DNA-PK (Ab-2) Mouse mAb (18-2) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Neutralization Studies, Paraffin Sections for the detection of DNA-PK (Ab-2).
免疫原
DNA-PK purified from HeLa cells
Epitope: within amino acids 1-2713 of DNA-PK
Human
應用
Frozen Sections (1:50-1:100)
Immunoblotting (1:250-1:500)
Immunofluorescence (1:50-1:100)
Immunoprecipitation (10 l/mg protein lysate)
Neutralization Studies (see comments)
Paraffin Sections (1:50-1:100, heat/pressure cooker pre-treatment required)
包裝
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
外觀
In 10 mM PBS, 0.2% BSA, pH 7.4.
重構
For long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
分析報告
Positive Control
A-431, HeLa, or HL-60 cells or normal tonsil, normal colon, or breast carcinoma tissue
A-431, HeLa, or HL-60 cells or normal tonsil, normal colon, or breast carcinoma tissue
其他說明
Kharbanda, S., et al. 1997. Nature386, 732.
McConnell, K.R., et al. 1997 J. Immunol.158, 2083-2089.
Peterson, S.R., et al. 1997. J. Biol. Chem.272, 10227.
Anderson, C.W. and Carter, T.H. 1996. Curr. Top. Microbiol. Immunol.217, 91.
Blunt, T., et al. 1996. Proc. Natl. Acad. Sci. USA93, 10285.
Carpenter, C.L. and Cantley, L.C. 1996. Curr. Opin. Cell. Biol.8, 153.
Chan, D.W. and Lees-Miller, S.P. 1996. J. Biol. Chem.271, 8936.
Connelly, M.A., et al. 1996. Gene175, 271.
Danska, J.S., et al. 1996. Mol. Cell. Biol.16, 5507.
Han, Z., et al. 1996. J. Biol. Chem.271, 25035.
Lees-Miller, S.P. 1996. Biochem. Cell. Biol.74, 503.
Song, Q., et al. 1996. EMBO J.15, 3238.
Teraoka, H., et al. 1996. FEBS Lett.393, 1.
Blunt, T., et al. 1995. Cell80, 813.
Hartley, K.O., et al. 1995. Cell82, 849.
Kirchgessner, C.U., et al. 1995. Science267, 1178.
Lees-Miller, S.P., et al. 1995. Science267, 1183.
Poltoratsky, V.P., et al. 1995. J. Immunol.155, 4529.
Anderson, C.W. 1993. Trends Biochem. Sci.18, 433.
Gottleib, T.M. and Jackson, S.P. 1993. Cell72, 131.
McConnell, K.R., et al. 1997 J. Immunol.158, 2083-2089.
Peterson, S.R., et al. 1997. J. Biol. Chem.272, 10227.
Anderson, C.W. and Carter, T.H. 1996. Curr. Top. Microbiol. Immunol.217, 91.
Blunt, T., et al. 1996. Proc. Natl. Acad. Sci. USA93, 10285.
Carpenter, C.L. and Cantley, L.C. 1996. Curr. Opin. Cell. Biol.8, 153.
Chan, D.W. and Lees-Miller, S.P. 1996. J. Biol. Chem.271, 8936.
Connelly, M.A., et al. 1996. Gene175, 271.
Danska, J.S., et al. 1996. Mol. Cell. Biol.16, 5507.
Han, Z., et al. 1996. J. Biol. Chem.271, 25035.
Lees-Miller, S.P. 1996. Biochem. Cell. Biol.74, 503.
Song, Q., et al. 1996. EMBO J.15, 3238.
Teraoka, H., et al. 1996. FEBS Lett.393, 1.
Blunt, T., et al. 1995. Cell80, 813.
Hartley, K.O., et al. 1995. Cell82, 849.
Kirchgessner, C.U., et al. 1995. Science267, 1178.
Lees-Miller, S.P., et al. 1995. Science267, 1183.
Poltoratsky, V.P., et al. 1995. J. Immunol.155, 4529.
Anderson, C.W. 1993. Trends Biochem. Sci.18, 433.
Gottleib, T.M. and Jackson, S.P. 1993. Cell72, 131.
This antibody will inhibit (approximately 50%) the kinase activity of DNA-PKcs. However, preincubation of the enzyme with DNA protects DNA-PKcs against inactivation by Clone 18-2. Antibody should be titrated for optimal results in individual systems.
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Tewodros Mamo et al.
Biochemical and biophysical research communications, 486(2), 307-313 (2017-03-17)
Osteosarcoma survival rate has not improved over the past three decades, and the debilitating side effects of the surgical treatment suggest the need for alternative local control approaches. Radiotherapy is largely ineffective in osteosarcoma, indicating a potential role for radiosensitizers.
Xiaosheng Wu et al.
Journal of immunology (Baltimore, Md. : 1950), 174(2), 934-941 (2005-01-07)
Activation-induced cytidine deaminase (AID) is required for Ig class switch recombination, a process that introduces DNA double-strand breaks in B cells. We show in this study that AID associates with the DNA-dependent protein kinase catalytic subunit (DNA-PKcs) promoting cell survival
Benu Brata Das et al.
The EMBO journal, 28(23), 3667-3680 (2009-10-24)
Human tyrosyl-DNA phosphodiesterase (TDP1) hydrolyzes the phosphodiester bond at a DNA 3' end linked to a tyrosyl moiety. This type of linkage is found at stalled topoisomerase I (Top1)-DNA covalent complexes, and TDP1 has been implicated in the repair of
Meena Shrivastav et al.
DNA repair, 8(8), 920-929 (2009-06-19)
DNA double-strand breaks (DSBs) are repaired by nonhomologous end-joining (NHEJ) and homologous recombination (HR). The NHEJ/HR decision is under complex regulation and involves DNA-dependent protein kinase (DNA-PKcs). HR is elevated in DNA-PKcs null cells, but suppressed by DNA-PKcs kinase inhibitors
Stéphanie Solier et al.
Molecular and cellular biology, 29(1), 68-82 (2008-10-29)
TRAIL is an endogenous death receptor ligand also used therapeutically because of its selective proapoptotic activity in cancer cells. In the present study, we examined chromatin alterations induced by TRAIL and show that TRAIL induces a rapid activation of DNA
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