AQ502B

Goat Anti-Mouse IgM+IgG+IgA Antibody, F(ab′)2, biotin conjugate, Species Adsorbed

0.5 mg/mL, Chemicon^®

• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.46

屬性

• 生物源: goat
• 品質等級: 100
• 共軛: biotin conjugate
• 抗體表格: purified antibody
• 抗體產品種類: secondary antibodies
• 無性繁殖: polyclonal
• 物種活性: mouse
• 製造商／商標名: Chemicon^®
• 濃度: 0.5 mg/mL
• 技術: ELISA: suitable; immunofluorescence: suitable
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified

描述

一般說明

IgG is the most abundant immunoglobulin and is comprised of two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.

特異性

Reacts with heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA. Absorbed for human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins.

The antibody reacts with the heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.

免疫原

Epitope: Mouse IgM, IgG, IgA

Prepared from pepsin digest of mouse Ig (H+L).

應用

Goat anti-Mouse IgM+IgG+IgA Antibody, F(ab′)2, biotin conjugate, Species Adsorbed detects level of Mouse IgM+IgG+IgA & has been published & validated for use in ELISA & IF.

Immunofluorescence: ≤ 1 μg/10⁶ cells.
ELISA: 1:5,000-1:20,000.

Optimal working dilutions must be determined by the end user.

Research Category
Secondary & Control Antibodies

Research Sub Category
Fragment Specific Secondary Antibodies

外觀

Gel filtration chromatography

Purified by gel filtration chromatography of pepsin digested antibody. Liquid in 1.0 mL PBS/NaN₃.

儲存和穩定性

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. Avoid repeated freeze/thaw cycles.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable