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ABS311

Sigma-Aldrich

Anti-LCN13 Antibody

serum, from rabbit

同義詞:

orant-binding protein 2a, Epididymal-specific lipocalin-13

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

serum

抗體產品種類

primary antibodies

無性繁殖

polyclonal

物種活性

mouse

技術

western blot: suitable

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

mouse ... Obp2A(227627)

一般說明

Epididymal-specific lipocalin-13 (LCN13) is also called Odorant-binding protein 2a (Obp2a). LCN13 is a secreted protein that is thought to act as a retinoid carrier protein within the epididymis, and have a role in male fertility. LCN13 is expressed in the epididymis.

特異性

This antibody detects two observed isoforms of LCN13.

免疫原

Recombinant protein corresponding to mouse LCN13.

應用

Detect Lipocalin using this rabbit polyclonal antibody, Anti-LCN13 Antibody validated for use in western blotting.
Research Category
Metabolism
Research Sub Category
Lipid Metabolism & Weight Regulation
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LCN13 in mouse lung tissue lysate.

Western Blotting Analysis: A representative lot from an independent laboratory detected LCN13 in liver, muscle, and pancreas tissues, but not in white adipose tissue, from LCN13 transgenic male mice (Cho, K. W., et al. (2011). Mol Cell Biol. 31(3):450-7.).

Western Blotting Analysis: A representative lot from an independent laboratory detected LCN13 in transfected mouse primary hepotcytes cell lysates (Sheng, L, et al. (2011). J Biol Chem. 286(44):38128-38135.).

Western Blotting Analysis: A representative lot detected LCN13 in mouse adipocyte cell lysates and in certain mouse organ tissues lysates (Zhou, Y. and Rui, L., (2013). Vitam Horm. 91:369-383.).

品質

Evaluated by Western Blotting in mouse skin tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LCN13 in 10 µg of LCN13 mouse skin tissue lysate.

標靶描述

~20 kDa observed. Two observed isoforms at ~20 kDa and ~28 kDa are likely due to proteolytic cleavage of a common LCN13 precursor (Cho, K. W., et al. (2011). Mol Cell Biol. 31(3):450-7.). Uncharacterized band(s) may be observed in some cell lysates.

外觀

Unpurified
Rabbit polyclonal serum with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析報告

Control
Mouse skin tissue lysate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1


分析證明 (COA)

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