推薦產品
生物源
rabbit
品質等級
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
純化經由
affinity chromatography
物種活性
rat, human
物種活性(以同源性預測)
chimpanzee (based on 100% sequence homology)
技術
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
ambient
目標翻譯後修改
unmodified
基因資訊
human ... LDHA(3939)
一般說明
L-lactate dehydrogenase A chain (EC 1.1.1.27; UniProt P00338; also known as Cell proliferation-inducing gene 19 protein, LDH muscle subunit, LDH-A, LDH-M, Renal carcinoma antigen NY-REN-59) is encoded by the LDHA (also known as GSD11, PIG19) gene (Gene ID 3939) in human. Originally described by Otto Warburg and now commonly known as the Warburg effect refers to the phenomenon that many tumor cells use aerobic glycolysis instead of mitochondrial respiration for energy production to support cell growth and proliferation. Enzymes involved in the complex network of metabolic pathways are therefore being studied as potential targets for cancer treatment. Lactate dehydrogenase (LDH) is a tetrameric enzyme composed of two different subunits, LDHA and LDHB, which can assemble into five different combinations (LDH1 is comprised of four LDHB subunits; LDH2 contains three LDHB and one LDHA subunit; LDH3 contains two LDHB and two LDHA subunits; LDH4 contains one LDHB and three LDHA subunits; LDH5 contains four LDHA subunits). LDHB is ubiquitously expressed and the predominant isoform in heart muscle, while LDHA is the predominant isoform in skeletal muscle and other highly glycolytic tissues. LDH catalyzes the reversible conversion of pyruvate into lactate. An upregulation of LDH therefore reduces the oxygen dependency and ensures an efficient anaerobic/glycolytic metabolism in tumor cells. Upregulated LDHA has been observed in hepatocellular carcinoma, breast, pancreatic, and prostate cancers. Inhibition of LDHA reduced cell malignant transformation and tumor formation. Serum LDHB level is also reported to elevate in lung cancer patients and progressively increase with the clinical stage of non-small cell lung cancer (NSCLC).
特異性
This rabbit polyclonal targeted epitope region is present in human LDHA spliced isoforms 1, 3, and 4, but absent from isoforms 2 and 5, reported by UniProt (P00338).
免疫原
KLH-conjugated linear peptide corresponding to a C-terminal region sequence of human LDHA.
應用
Anti-LDHA, Cat. No. ABN896, is a highly specific rabbit polyclonal antibody, that targets L-lactate dehydrogenase A chain and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected LDHA in HUVECs and HeLa cells.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected LDHA in rat testis, human prostate cancer and kidney tissue sections.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected LDHA in rat testis, human prostate cancer and kidney tissue sections.
Research Category
Neuroscience
Neuroscience
品質
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected LDHA in 10 µg of HepG2 cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected LDHA in 10 µg of HepG2 cell lysate.
標靶描述
~37 kDa observed. 36.56/39.71/30.07 kDa (human isoform 1/3/4) and 36.32 kDa (rat) calculated (Met1 removed). Uncharacterized band(s) may appear in some lysates.
聯結
Replaces: ABN311
外觀
Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
其他說明
Concentration: Please refer to lot specific datasheet.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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