重組細胞
expressed in baculovirus infected Sf21 cells
品質等級
描述
protein target PI3K-C2α
純化經由
immobilized metal affinity chromatography (IMAC)
技術
activity assay: suitable
UniProt登錄號
基因資訊
human ... PIK3CG(5294)
一般說明
N-terminal 6His-tagged recombinant human PI3K-C2α amino acids 299–end.
包裝
Also available in 250μg size as catalog # 14-906M - Call for pricing and availability.
品質
Routinely evaluated in the PIProfiler.
物理性質
161kDa
外觀
10 μg of enzyme in 10.6 μl of 50 mM Tris/HCl, pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 0.2 mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol, 20 mM β-glycerophosphate, 0.25 mM Na3VO4, 10 mM NaF. Frozen solution.
儲存和穩定性
6 months at -70°C
分析報告
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
其他說明
FUNCTION: Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2. May play a role in clathrin-coated endocytic vesicle formation and EGF signaling cascade. May be involved in mitosis and UV-induced damage response. May be a downstream effector in insulin signaling cascade.
CATALYTIC ACTIVITY: ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate.
COFACTOR: Calcium or magnesium. Manganese cannot be used.
ENZYME REGULATION: Activated by insulin. Only slightly inhibited by wortmannin and LY294002. Activated by clathrin.
Subunit structure: Part of a complex with ERBB2 and EGFR. Interacts with clathrin trimers.
SUBCELLULAR LOCATION: Cell membrane. Golgi apparatus. Cytoplasmic vesicle › clathrin-coated vesicle. Nucleus. Cytoplasm. Associated with RNA-containing structures.
TISSUE SPECIFICITY: Expressed in columnar and transitional epithelia, mononuclear cells, smooth muscle cells, and endothelial cells lining capillaries and small venules (at protein level). Ubiquitously expressed, with highest levels in heart, placenta and ovary, and lowest levels in the kidney.
SIMILARITY: Belongs to the PI3/PI4-kinase family. Contains 1 C2 domain. Contains 1 PI3K/PI4K domain. Contains 1 PX (phox homology) domain.
CATALYTIC ACTIVITY: ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate.
COFACTOR: Calcium or magnesium. Manganese cannot be used.
ENZYME REGULATION: Activated by insulin. Only slightly inhibited by wortmannin and LY294002. Activated by clathrin.
Subunit structure: Part of a complex with ERBB2 and EGFR. Interacts with clathrin trimers.
SUBCELLULAR LOCATION: Cell membrane. Golgi apparatus. Cytoplasmic vesicle › clathrin-coated vesicle. Nucleus. Cytoplasm. Associated with RNA-containing structures.
TISSUE SPECIFICITY: Expressed in columnar and transitional epithelia, mononuclear cells, smooth muscle cells, and endothelial cells lining capillaries and small venules (at protein level). Ubiquitously expressed, with highest levels in heart, placenta and ovary, and lowest levels in the kidney.
SIMILARITY: Belongs to the PI3/PI4-kinase family. Contains 1 C2 domain. Contains 1 PI3K/PI4K domain. Contains 1 PX (phox homology) domain.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
訊號詞
Warning
危險聲明
危險分類
Skin Sens. 1
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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