1.25062

MiniChrom Column Fractogel COO (M), 1 ml

MiniChrom Fractogel^® COO (M), volume 1 mL, prepacked column

• 同義詞: Fractogel^® EMD COO^- (M)
• 分類程式碼代碼: 41115711

屬性

• ligand: (Carboxyethyl )
• 品質等級: 100
• 材料: polypropylene housing
• 產品線: Fractogel^®
• 形狀: prepacked column
• 參數: ≤8 bar pressure; ~300 cm/hr max. flow rate
• 柱內徑 × 長度: 8 mm × 20 mm
• 容積: 1 mL
• 基質活性組: Methacrylate
• 平均粒徑: 40-90 μm
• 容量: ~100 mL, resin binding capacity
• 應用: mAb; recombinant protein; vaccine development
• 分離技術: cation exchange
• 儲存溫度: 15-25°C

描述

一般說明

8 x 20mm

特點和優勢

MiniChrom Column Fractogel^® COO (M), 1 ml enables:

• Good resolution between monomers and aggregates
• Efficient aggregate and HCP removal combined with high yield
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior, the ion exchange capacity can be used from pH 6 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel^® EMD COO^- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the degree of the ionization of the functional groups of the exchanger
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

分析報告

Appearance: passes test

法律資訊

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany