07-425-I

Anti-Neurogranin

from rabbit, purified by affinity chromatography

• 同義詞: Ng, RC3
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: rat, human
• 物種活性（以同源性預測）: goat (based on 100% sequence homology), bovine (based on 100% sequence homology), mouse (based on 100% sequence homology)
• 包裝: antibody small pack of 25 μg
• 技術: immunohistochemistry: suitable (paraffin); western blot: suitable
• 同型: IgG
• NCBI登錄號: NP_001119653
• UniProt登錄號: Q92686
• 目標翻譯後修改: unmodified
• 基因資訊: human ... NRGN(4900)

描述

一般說明

Neurogranin (UniProt: Q92686; also known as Ng, RC3) is encoded by the NRGN gene (Gene ID: 4900) in human. Neurogranin is abundantly expressed in cerebral cortex, hippocampus, amygdala, and striatum. In neurons, it is found concentrated at dendritic spines. It acts as a third messenger and serves as a substrate for protein kinase C-mediated molecular cascades during synaptic development and remodeling. It contains an IQ domain (aa 26-347) and a collagen-like domain (aa 48-78). The IQ domain mediates its interaction with calmodulin and phosphatidic acid (PA). Neurogranin is intrinsically unstructured, however, upon binding with calmodulin, the IQ domain adopts a helical conformation. Neurogranin is phosphorylated at serine 36 by protein kinase C and following phosphorylation neurogranin is unable to bind either calmodulin or PA, which can lead to decline in memory-associated functions. Higher levels of neurogranin have also been reported in brains of elderly and is considered a factor in memory decline, independent of Alzheimer s disease. (Ref.: Javier Dı´ez-Guerra, F. (2010). Life. 62(8): 597-606; Casaletto, KB et al. (2017). Neurology 89(17); 1782-1788).

特異性

This rabbit polyclonal antibody sepecifically detects Neurogranin. It targets an epitope with in 12 amino acids from the C-terminal region.

免疫原

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal region of human Neurogranin.

應用

Anti-Neurogranin Antibody, Cat. No. 07-425-I, is a highly specific rabbit polyclonal antibody that targets Neurogranin and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Neurogranin in human and rat brain tissue sections.

Research Category
Neuroscience

品質

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Neurogranin in rat brain tissue lysate.

標靶描述

~15 kDa observed; 7.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.