Skip to Content
Merck
  • Serum 7 alpha-hydroxy-4-cholesten-3-one and selenohomocholyltaurine (SeHCAT) whole body retention in the assessment of bile acid induced diarrhoea.

Serum 7 alpha-hydroxy-4-cholesten-3-one and selenohomocholyltaurine (SeHCAT) whole body retention in the assessment of bile acid induced diarrhoea.

European journal of gastroenterology & hepatology (1996-02-01)
W G Brydon, H Nyhlin, M A Eastwood, M V Merrick
ABSTRACT

To assess the reliability of serum 7 alpha-hydroxy-4-cholesten-3-one (7 alpha-3ox-C) in the differential diagnosis of bile acid induced diarrhoea by comparison with 75selenohomocholyltaurine whole body retention (SeHCAT WBR). One hundred and sixty-four patients with chronic diarrhoea were investigated prospectively in two centres (Edinburgh and Sweden) by two different tests which measure bile acid loss or synthesis: the SeHCAT test which measures the 7-day SeHCAT WBR and serum 7 alpha-3ox-C which reflects the rate of bile acid synthesis. Forty-six patients had SeHCAT WBR of less than 10% (19 with ileal disease or resection, nine with idiopathic bile acid induced diarrhoea and 18 with miscellaneous causes for bile acid induced diarrhoea). All patients with ileal or idiopathic disease showed a favorable response to treatment as did 13 of the miscellaneous group. Serum 7 alpha-3ox-C was raised in all subjects with ileal disease/resection, seven patients with idiopathic disease and all subjects in the miscellaneous group who responded to treatment. Sixteen out of 118 patients with SeHCAT WBR greater than or equal to 10% had raised serum 7 alpha-3ox-C. The positive predictive value of serum 7 alpha-3ox-C was 74%. The high negative predictive value (98%) of serum 7 alpha-3ox-C indicates the possible use of this test for excluding bile acid malabsorption in this population. All but two subjects who responded to treatment had raised serum 7 alpha-3ox-C concentrations. The possibility that the sensitivity of the test can be improved by repeat testing needs to be further investigated. There was a significant correlation between fractional catabolic rate (FCR) SeHCAT and serum 7 alpha-3ox-C (r = 0.63, P < 0.0001). Further data are required to validate the reference range in women over 70 years of age.

MATERIALS
Product Number
Brand
Product Description

Supelco
Nicotinic acid, analytical standard
Pricing and availability is not currently available.
Sigma-Aldrich
Isopropyl alcohol, ≥99.7%, FCC, FG
Pricing and availability is not currently available.
Sigma-Aldrich
Reagent Alcohol, reagent grade
Pricing and availability is not currently available.
Supelco
Progesterone, VETRANAL®, analytical standard
Pricing and availability is not currently available.
Sigma-Aldrich
Acetaldehyde solution, natural, 50 wt. % ethanol, FG
Pricing and availability is not currently available.
Sigma-Aldrich
Acetone, ≥99%, meets FCC analytical specifications
Pricing and availability is not currently available.
Sigma-Aldrich
Reagent Alcohol, anhydrous, ≤0.003% water
Pricing and availability is not currently available.
Sigma-Aldrich
Nicotinic acid, ≥99.5% (HPLC)
Pricing and availability is not currently available.
Sigma-Aldrich
Nicotinic acid, meets USP testing specifications
Pricing and availability is not currently available.
Sigma-Aldrich
Nicotinic acid, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥98%
Pricing and availability is not currently available.
Sigma-Aldrich
Ethanol Fixative 80% v/v, suitable for fixing solution (blood films)
Pricing and availability is not currently available.
Sigma-Aldrich
Nicotinic acid, ≥98%
Pricing and availability is not currently available.
Sigma-Aldrich
Reagent Alcohol, anhydrous, ≤0.005% water
Pricing and availability is not currently available.
Sigma-Aldrich
Progesterone, powder, BioReagent, suitable for cell culture
Pricing and availability is not currently available.
Sigma-Aldrich
Progesterone, γ-irradiated, BioXtra, suitable for cell culture
Pricing and availability is not currently available.
Sigma-Aldrich
Progesterone, meets USP testing specifications
Pricing and availability is not currently available.
Sigma-Aldrich
Acetaldehyde solution, 5 M in THF
Pricing and availability is not currently available.
Supelco
Acetone, analytical standard
Pricing and availability is not currently available.
Supelco
Dehydrated Alcohol, Pharmaceutical Secondary Standard; Certified Reference Material
Pricing and availability is not currently available.
Supelco
5α-Dihydrotestosterone (DHT) solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
Pricing and availability is not currently available.
Sigma-Aldrich
Acetaldehyde solution, 40 wt. % in H2O
Pricing and availability is not currently available.
Supelco
Progesterone, Pharmaceutical Secondary Standard; Certified Reference Material
Pricing and availability is not currently available.
Supelco
Acetone, Pharmaceutical Secondary Standard; Certified Reference Material
Pricing and availability is not currently available.
Supelco
Niacin (Nicotinic Acid), Pharmaceutical Secondary Standard; Certified Reference Material
Pricing and availability is not currently available.
Supelco
7α-Hydroxy-4-cholesten-3-one, ≥95.0% (HPLC)
Pricing and availability is not currently available.
Sigma-Aldrich
Acetone, puriss., meets analytical specification of Ph. Eur., BP, NF, ≥99% (GC)
Pricing and availability is not currently available.
Sigma-Aldrich
Acetone, ACS reagent, ≥99.5%
Pricing and availability is not currently available.
Sigma-Aldrich
Acetone, Laboratory Reagent, ≥99.5%
Pricing and availability is not currently available.
Sigma-Aldrich
Acetone, ACS reagent, ≥99.5%
Pricing and availability is not currently available.
Sigma-Aldrich
Acetone, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)
Pricing and availability is not currently available.