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Potassium ion efflux induced by cationic compounds in yeast.

Biochimica et biophysica acta (1999-04-21)
E Enríquez-Freire, R López, A Peña
ABSTRACT

Potassium efflux in yeast induced by several cationic compounds showed different characteristics. All of the observed efflux required glucose as substrate at the concentrations used. For most of them, the phenomenon required binding of the cationic compound to the cell surface and increased with the negative cell surface charge, and for all the compounds tested, it depended on a metabolizable substrate. Efflux induced with terbium chloride appeared more likely due to the function of a K+/H+ antiporter. With DEAE-dextran and dihydrostreptomycin, potassium efflux was dependent on the cell potassium content and was also sensitive to osmotic changes of the medium. DEAE-dextran-provoked efflux was not due to cell disruption. Dihydrostreptomycin seemed to activate a potassium efflux system which could not be studied in isolation, but its inhibition of potassium uptake may also be involved. Except for cells treated with ethidium bromide, no appreciable cell disruption was observed. The potassium efflux observed appears to be a membrane phenomenon reversible after washing with magnesium chloride.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Terbium(III) chloride, anhydrous, powder, 99.9% trace metals basis
Sigma-Aldrich
Terbium(III) chloride hexahydrate, 99.9% trace metals basis
Sigma-Aldrich
Terbium(III) chloride, anhydrous, powder, 99.99% trace metals basis
Sigma-Aldrich
Terbium(III) chloride hexahydrate, 99.999% trace metals basis