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Supelco

SUPELCOSIL™ LC-18 (5 µm) HPLC Columns

L × I.D. 2 cm × 4 mm Supelguard Guard Cartridge pkg of 2 ea, Guard Cartridge holder required for use

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501

product name

SUPELCOSIL™ LC-18 Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm

Agency

suitable for USP L1

packaging

pkg of 1 kit

parameter

<70 °C temp. range

technique(s)

HPLC: suitable

L × I.D.

2 cm × 4 mm

matrix

silica gel high purity, spherical

matrix active group

C18 (octadecyl) phase

particle size

5 μm

pore size

120 Å

pH range

2-7.5

application(s)

food and beverages

compatibility

use to protect LC-18, LC-PAH

separation technique

reversed phase

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Application

SUPELCOSIL™ LC-18 Supelguard Cartridge may be used as a guard column along with SUPELCOSIL™ LC-18 HPLC Column during the analysis of:
  • Synthetic food dyes in commercial soft drinks by ion-pair high performance liquid chromatography (HPLC IP).
  • Drugs in plasma and brain samples by solid-phase extraction (SPE) and protein precipitation in conjunction with HPLC combined with ultraviolet (UV) detection.

Footnote

Kit includes one cartridge, a stand-alone holder, a piece of tubing, and 2 nuts and ferrules.

Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

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Practice of solid-phase extraction and protein precipitation in the 96-well format combined with high-performance liquid chromatography-ultraviolet detection for the analysis of drugs in plasma and brain.
Rouan MC, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 754(1), 45-55 (2001)
Determination of synthetic food dyes in commercial soft drinks by TLC and ion-pair HPLC.
de Andrade FI, et al.
Food Chemistry, 157, 193-198 (2014)
K M Matar et al.
Therapeutic drug monitoring, 21(5), 559-566 (1999-10-16)
A simple, rapid, sensitive, and reproducible high-performance liquid chromatographic (HPLC) method for simultaneous determination of the antiepileptic drugs (ethosuximide, primidone, lamotrigine, phenobarbital, phenytoin, and carbamazepine) and two metabolites (carbamazepine-diol and carbamazepine-epoxide) in human plasma is described. The procedure involves extraction
Carola F Ferreyra et al.
Journal of AOAC International, 88(6), 1649-1654 (2006-03-11)
The aim of this research was to develop and validate a sensitive, rapid, easy, and precise reversed-phase liquid chromatography (LC) method for stability studies of bifonazole (I) formulated with tinctures of calendula flower (II). The method was especially developed for
Khaled S Abdelkawy et al.
Biomedical chromatography : BMC, 31(3) (2016-08-25)
A simple, accurate, and reproducible high-performance liquid chromatography (HPLC) method has been developed and validated for the quantification of quercetin (QR) in rat plasma. The method involves a simple protein precipitation procedure to extract both QR and thymoquinone (TQ), the
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