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SRP0342

Sigma-Aldrich

cKit active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥32% (SDS-PAGE)

Synonym(s):

CD117, KIT, PBT, SCFR, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog

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About This Item

CAS Number:
UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected Sf9 cells

Assay

≥32% (SDS-PAGE)

form

aqueous solution

mol wt

53.5 kDa

packaging

pkg of 10 μg

technique(s)

activity assay: suitable
inhibition assay: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... KIT(3815)

General description

Human cKit (D816V) ( GenBank Accession No. NM_000222), amino acids 544-end with N-terminal His-tag, MW=50 kDa, expressed in an Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Physical form

Fomulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 3 mM DTT and 10% glycerol.

Pictograms

Health hazardExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Y Yarden et al.
The EMBO journal, 6(11), 3341-3351 (1987-11-01)
Structural features of v-kit, the oncogene of HZ4 feline sarcoma virus, suggested that this gene arose by transduction and truncation of cellular sequences. Complementary DNA cloning of the human proto-oncogene coding for a receptor tyrosine kinase confirmed this possibility: c-kit
Mauro Picardo et al.
The Journal of investigative dermatology, 131(6), 1182-1185 (2011-05-14)
Cutaneous pigmentation is regulated by a complex melanogenic network in which skin cells synthesize growth factors and cytokines. Mutations in genes encoding these regulators modify their expression and/or functionality, leading to altered signaling pathways and contributing to altered skin phenotypes.

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