MABS1688

Anti-NCoA-2 Antibody, clone 1C6.3

clone 1C6.3, from mouse

• Synonym(s): Nuclear receptor coactivator 2, bHLHe75, Class E basic helix-loop-helix protein 75, hTIF2, NCoA-2, Transcriptional intermediary factor 2
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 1C6.3, monoclonal
• species reactivity: human
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_059111
• UniProt accession no.: Q15596
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... NCOA2(10499)

Description

General description

Nuclear receptor coactivator 2 (UniProt Q15596; also known as bHLHe75, Class E basic helix-loop-helix protein 75, hTIF2, NCoA-2, Transcriptional intermediary factor 2) is encoded by the NCOA2 (also known as BHLHE75, SRC2, TIF2) gene (Gene ID 10499) in human. Nuclear receptor coactivator 2 (NCoA-2) is a 160 kDa transcriptional coactivator for steroid receptors and nuclear receptors that acts as a coactivator of the steroid binding domain (AF-2), but not the modulating N-terminal domain (AF-1). It possesses a single NR interaction domain (NID) and two autonomous activation domains, AD1 and AD2. NCoA-2 assists nuclear receptors in the upregulation of DNA expression. In cells ligand activated nuclear receptors recruit NCoA-2 to DNA where it acylates histones to make targeted regions accessible to transcriptional machinery. Along with NCOA1 it controls energy balance between white and brown adipose tissues and also acts as a critical regulator of glucose metabolism. It is also involved in the positive regulation of the transcriptional activity of the glucocorticoid receptor NR3C1 by sumoylation enhancer RWDD3. NCoA-2 is reported to positively regulate the circadian clock by acting as a transcriptional coactivator for the CLOCK-ARNTL/BMAL1 heterodimer. Chromosomal aberrations involving NCOA2 may be a cause of acute myeloid leukemias.

Ref.: Voegel, JJ et al. (1998). EMBO J. 17, 507 519.

Specificity

Clone 1C6.3 targeted epitope lies within the C-terminal one fourth region of the full-length human NCoA-2.

Immunogen

GST-tagged recombinant human NCoA-2 C-terminal region fragment..

Application

Detect NCOA2 using this mouse monoclonal Anti-NCoA-2 Antibody, clone 1C6.3, Cat. No. MABS1688, validated for use in Immunohistochemistry (Paraffin) and Western blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected nuclear NCoA-2 immunoreactivity in human lymphoma, ovarian cancer, and small intestine tissue sections.

Quality

Evaluated by Western Blotting in human osteosarcoma cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected NCoA-2 in 10 µg of U2OS human osteosarcoma cell lysate.

Target description

~160 kDa observed. 159.0 kDa (Met1 removed) calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1