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Merck

Pretreatment of Agave americana stalk for enzymatic saccharification.

Bioresource technology (2012-11-06)
Qiang Yang, Xuejun Pan
ABSTRACT

Agave americana is one of commonly grown agave species but currently less valuable because its large flower stalk cannot be used for producing alcoholic beverage. In the present study, the stalk was pretreated with dilute acid (DA), sulfite (SPORL), and sodium hydroxide (NaOH) to preliminarily assess its potential as feedstock for bioethanol production. The changes of cell wall components during the pretreatments, enzymatic digestibility of the pretreated stalks, and the adsorption of cellulases on the substrates were investigated. Results indicated that the pretreatments significantly improved the enzymatic digestibility of the agave stalk. SPORL pretreatment gave higher substrate and sugar yields, while NaOH pretreated stalk had better digestibility under the investigated conditions. The better hydrolysability of NaOH-pretreated stalk was attributed to low lignin and hemicellulose content and high affinity to cellulases.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sodium sulfite, puriss. p.a., reag. Ph. Eur., anhydrous, 98-100.0% (iodometric)
Sigma-Aldrich
Sodium sulfite, ACS reagent, ≥98.0%
Sigma-Aldrich
Sodium sulfite, JIS special grade, ≥97.0%
Sigma-Aldrich
Sodium sulfite, BioXtra, ≥98%
Sigma-Aldrich
Sodium sulfite, puriss., meets analytical specification of Ph. Eur., BP, NF, anhydrous, 95.0-100.0% (iodometric)
Sigma-Aldrich
Sodium sulfite, ≥98%
Sigma-Aldrich
Sodium sulfite, anhydrous, Redi-Dri, reagent grade, ≥98%
Sigma-Aldrich
Sodium sulfite, BioUltra, anhydrous, ≥98% (RT)