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H6269

Sigma-Aldrich

Hexadecyltrimethylammonium bromide

for molecular biology, ≥99%

Synonym(s):

CTAB, Cetrimonium bromide, Cetyltrimethylammonium bromide, Palmityltrimethylammonium bromide

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About This Item

Linear Formula:
CH3(CH2)15N(Br)(CH3)3
CAS Number:
57-09-0
Molecular Weight:
364.45
Beilstein:
3598189
EC Number:
200-311-3
MDL number:
MFCD00011772
UNSPSC Code:
12161902
PubChem Substance ID:
24895734
NACRES:
NA.31

grade

for molecular biology

description

cationic

Assay

≥99%

form

powder or crystals

mol wt

micellar avg mol wt 62,000

aggregation number

170

pH

5.0-7 (25 °C, 36.4 g/L)

CMC

0.92
1 mM (20-25°C)

mp

248-251 °C (lit.)

solubility

H2O: 1/10 g/mL

HLB

10

suitability

suitable for DNA precipitation

foreign activity

DNAse (Detection)
NICKase Detection
RNAse, none detected

SMILES string

[Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C

InChI

1S/C19H42N.BrH/c1-5-6-7-8-9-10-11-12-13-14-15-16-17-18-19-20(2,3)4;/h5-19H2,1-4H3;1H/q+1;/p-1

InChI key

LZZYPRNAOMGNLH-UHFFFAOYSA-M

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Application

Hexadecyltrimethylammonium bromide has been used:

  • as a component of DNA extraction buffer and RNA extraction buffer
  • as a constituent of dye dissolution buffer

Biochem/physiol Actions

Hexadecyltrimethylammonium bromide also called cetyltrimethylammonium bromide (CTAB), is a cationic surfactant. CTAB method is an efficient, cost and time effective method of DNA extraction.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2 - STOT RE 2 Oral - STOT SE 3

Target Organs

Gastrointestinal tract, Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

471.2 °F - closed cup

Flash Point(C)

244 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Weijun Xu et al.
Materials science & engineering. C, Materials for biological applications, 103, 109854-109854 (2019-07-28)
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The importance of the CTAB surfactant on the colloidal seed-mediated synthesis of gold nanorods
Smith DK and Korgel BA
Langmuir, 24(3), 644-649 (2008)
Investigation of the Orientation of C T A
Karaca S, et al.
Journal of Chemistry, 2013 (2012)
Xanthoferrin Siderophore Estimation from the Cell-free Culture Supernatants of Different Xanthomonas Strains by HPLC
Pandey SS, et al.
Archives of Virology, 7, e2410-e2410 (2017)
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