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F3639

Sigma-Aldrich

4-Fluoro-7-sulfamoylbenzofurazan

Synonym(s):

4-(Aminosulfonyl)-7-fluorobenzofurazane, 7-Fluoro-2,1,3-benzoxadiazole-4-sulfonamide, 7-Fluorobenzofurazan-4-sulfonamide, ABD-F

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About This Item

Empirical Formula (Hill Notation):
C6H4FN3O3S
CAS Number:
Molecular Weight:
217.18
Beilstein:
5035147
MDL number:
UNSPSC Code:
12352108
PubChem Substance ID:
NACRES:
NA.32

storage temp.

−20°C

SMILES string

NS(=O)(=O)c1ccc(F)c2nonc12

InChI

1S/C6H4FN3O3S/c7-3-1-2-4(14(8,11)12)6-5(3)9-13-10-6/h1-2H,(H2,8,11,12)

InChI key

XROXHZMRDABMHS-UHFFFAOYSA-N

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General description

4-Fluoro-7-sulfamoylbenzofurazan is a fluorescent reagent, which directly links to the sulfur atom of thiols without a flexible alkyl chain. It is used to detect low molecular thiols using high performance liquid chromatography.

Application

Reagent for fluorimetric assay of thiols.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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C C Chin et al.
Analytical biochemistry, 214(1), 128-134 (1993-10-01)
The use of the reagent tributyl phosphine (Bu3P) to reduce disulfides (Ruegg, U.T., and Rudinger, J., Methods Enzymol. 47, 111-116, 1977) and of 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (ABD-F) to block free sulphydryl groups (Toyo'oka, T., and Imai, K. Anal. Chem. 56, 2461-2464, 1984)
K Imai et al.
Biomedical chromatography : BMC, 7(5), 275-276 (1993-09-01)
L- and D-Amino acids (Leu or Phe) were derivatized with fluorogenic reagents, 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), 4-(N,N-dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (DBD-F), 4-aminosulfonyl-7-fluoro-2,1,3-benzoxadiazole (ABD-F) and 5-(N,N-dimethylamino)naphthalene-1-sulfonylchloride (DNS-CI), and separated on a Pirkle type column, Sumichiral OA 2500 (S) ((S)-1-naphthylglycyl-3,5-dinitrophenylamide silica gel) with a mobile phase of
Y Luo et al.
The Journal of biological chemistry, 274(25), 17733-17741 (1999-06-11)
Serpins form enzymatically inactive covalent complexes (designated E*I*) with their target proteinases, corresponding most likely to the acyl enzyme that resembles the normal intermediate in substrate turnover. Formation of E*I* involves large changes in the conformation of the reactive center
Mikhail Linetsky et al.
Investigative ophthalmology & visual science, 44(9), 3920-3926 (2003-08-27)
To determine the mechanism that leads to the UVA light-dependent loss of glutathione reductase (GR) activity in human lens (HL). Both the HL water-soluble (WS) fraction and yeast GR were irradiated with UVA light (200 mW/(cm(2). h) for 1 hour
S Maruta et al.
Journal of biochemistry, 128(4), 687-694 (2000-09-30)
In the presence of excess amounts of fluorine, a physiological divalent cation, magnesium (Mg(2+)), forms a novel phosphate analogue, magnesium fluoride (MgFn). Park et al. [Biochim. Biophys. Acta 1430, 127-140 (1999)] previously demonstrated that MgADP. MgFn forms a complex with

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