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Key Documents

07-330

Sigma-Aldrich

Anti-Partitioning-defective 3 Antibody

Upstate®, from rabbit

Synonym(s):

Atypical PKC isotype-specific-interacting protein, CTCL tumor antigen se2-5, atypical PKC isotype-specific interacting protein, par-3 (partitioning defective 3, C.elegans) homolog, par-3 partitioning defective 3 homolog (C. elegans), partitioning-defecti

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, canine, human, mouse, monkey

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

General description

PAR3 is an adapter protein involved in asymmetrical cell division and cell polarization processes. It seems to play a central role in the formation of epithelial tight junctions. Its association with PARD6B may prevent the interaction of PAR3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PAR3 complex links GTP bound Rho small GTPases to atypical protein kinase C proteins. PAR3 interacts with PARD6A and PARD6B. Isoform 2, but not at least isoform 3 interacts with PRKCZ. PAR3 interacts with PRCKI. PAR3 forms part of a complex with PARD6A or PARD6B, PRKCI or PRKCZ and CDC42 or RAC1. PAR3 interacts with F11R/JAM1. Antibodies against PAR3 are present in sera from patients with cutaneous T cell lymphomas.

Specificity

Recognizes all 3 slice isoforms of PAR-3, MW ~180, 150, 100 kDa.

Immunogen

GST fusion protein corresponding to residues 457-744 of mouse PAR-3 (accession number AY02657).

Application

Anti-Partitioning-defective 3 Antibody is an antibody against Partitioning-defective 3 and has been published in over 20 citations for use in IC and WB.
Immunocytochemistry:
A previous lot of this antibody showed positive immunostaining for PAR-3 in MDCK cells fixed with 3% paraformaldehyde followed by permeabilization with 0.1% Triton X-100 has been shown by an independent laboratory.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton

Quality

Evaluated by western blot on RIPA lysates from 3T3/A31, A431, and HeLa cells.

Western Blot Analysis:
0.5-2 µg/mL of this antibody detected PAR-3 isoforms in RIPA lysates from 3T3/A31, A431, and HeLa cells.

Target description

~180, 150 & 100 kDa

Linkage

Replaces: MABS174

Physical form

Affinity Purified
Format: Purified
Purified rabbit polyclonal in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Junctional adhesion molecules (JAMs): more molecules with dual functions?
Ebnet, Klaus, et al.
Journal of Cell Science, 117, 19-29 (2004)
Cdx2 regulates endo-lysosomal function and epithelial cell polarity.
Gao N, Kaestner KH
Genes & Development null
hnRNP K post-transcriptionally co-regulates multiple cytoskeletal genes needed for axonogenesis.
Liu, Y; Szaro, BG
Development null
PKC? mediates breakdown of outer blood-retinal barriers in diabetic retinopathy.
Omri, S; Behar-Cohen, F; Rothschild, PR; Gelize, E; Jonet, L; Jeanny, JC; Omri, B; Crisanti, P
Testing null
ASPP2 regulates epithelial cell polarity through the PAR complex.
Weili Cong,Tomonori Hirose,Yutaka Harita,Akio Yamashita,Keiko Mizuno,Hisashi Hirano,Shigeo Ohno
Current Biology null

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