biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
4A4, monoclonal
mol wt
calculated mol wt 24.81 kDa
observed mol wt ~35 kDa
purified by
using protein G
species reactivity
virus
packaging
antibody small pack of 100 μL
technique(s)
ELISA: suitable
electron microscopy: suitable
immunocytochemistry: suitable
inhibition assay: suitable
western blot: suitable
isotype
IgG1κ
epitope sequence
Unknown
Protein ID accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
vaccinia virus ... K8.1(4961469)
General description
Kaposi′s sarcoma-associated herpesvirus glycoprotein K8.1 (UniProt: O36551; also known as Glycoprotein K8.1, Glycoprotein K8.1A, Glycoprotein gp35/37, K8.1, K8.1 protein) is encoded by the K8.1 (also known as HHV8GK18_gp55) gene (Gene ID: 4961469) in Kaposi′s sarcoma-associated herpesvirus (KSHV). Kaposi sarcoma virus is an oncogenic human g-herpesvirus that has been identified in AIDS patients in association with Kaposi′s sarcoma. It can persist latently in host cells while expressing only a limited set of viral genes and maintaining its genome as extrachromosomal plasmids. This virus encodes two immunogenic class I glycoproteins, K8.1A and K8.1B. These glycoproteins have several serine and threonine residues that serves as potential sites for O-glycosylation. The 228-amino-acid-long K8.1A open reading frame (ORF) contains four N-glycosylation sites and the 167-amino-acid-long K8.1B ORF contains three N-glycosylation sites. Glycoprotein K8.1A is reported to mediate KSHV binding to target cells via interaction with heparan sulfate. This protein is involved in binding to target cells but is not required for viral entry into cells. Clone 4A4 detects both K8.1A and K8.1B glycoprotein and is shown to react with infected cell membranes and virion envelope. This antibody is shown to partially inhibit KSHV infection in HEK293 cells. (Ref.: Faure, A., et al. (2019). Proc. Natl. Acad. Sci. USA. 116(33); 16519-16528; Zhu, L., et al. (1999). Virology 262(1), 237-249).
Specificity
Clone 4A4 is a mouse monoclonal antibody that detects Glycoprotein K8.1 of Kaposi′s sarcoma-associated herpesvirus.
Immunogen
Phorbol-12-myristate-13-acetate TPA))- induced BCBL-1 (Body Cavity Based Lymphoma-1) cells.
Application
Quality Control Testing
Evaluated by Western Blotting in Phorbol-12-myristate-13-acetate (TPA)-induced BCBL-1 cells.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Kaposi′s sarcoma-associated herpesvirus (KSHV) in Phorbol-12-myristate-13-acetate (TPA)- induced BCBL-1 cells.
Tested Applications
Inhibition: A representative lot of this antibody inhibited infection by KSHV in HEK293 cells.(Faure, A., et al. (2019). Proc Natl Acad Sci USA. 116(33):16519-16528).
Electron Microscopy: A representative lot of this antibody detected K8.1A glycoprotein in TPA stimulated BCBL-1 cells. (Zhu, L., et al. (1999). Virology. 262(1):237-49).
Western Blotting Analysis: A representative lot detected KSHV gpK8.1A in Western Blotting applications (Zhu, L., et al. (1999). Virology. 262(1):237-49).
ELISA Analysis: A representative lot detected KSHV gpK8.1A in ELISA applications (Zhu, L., et al. (1999). Virology. 262(1):237-49).
Immunocytochemistry Analysis: A representative lot detected KSHV gpK8.1A in Immunocytochemistry applications (Zhu, L., et al. (1999). Virology. 262(1):237-49).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Phorbol-12-myristate-13-acetate (TPA)-induced BCBL-1 cells.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Kaposi′s sarcoma-associated herpesvirus (KSHV) in Phorbol-12-myristate-13-acetate (TPA)- induced BCBL-1 cells.
Tested Applications
Inhibition: A representative lot of this antibody inhibited infection by KSHV in HEK293 cells.(Faure, A., et al. (2019). Proc Natl Acad Sci USA. 116(33):16519-16528).
Electron Microscopy: A representative lot of this antibody detected K8.1A glycoprotein in TPA stimulated BCBL-1 cells. (Zhu, L., et al. (1999). Virology. 262(1):237-49).
Western Blotting Analysis: A representative lot detected KSHV gpK8.1A in Western Blotting applications (Zhu, L., et al. (1999). Virology. 262(1):237-49).
ELISA Analysis: A representative lot detected KSHV gpK8.1A in ELISA applications (Zhu, L., et al. (1999). Virology. 262(1):237-49).
Immunocytochemistry Analysis: A representative lot detected KSHV gpK8.1A in Immunocytochemistry applications (Zhu, L., et al. (1999). Virology. 262(1):237-49).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-KSHV gpK8.1A, clone 4A4, Cat. No. MABF2678, is a mouse monoclonal antibody that detects Kaposi′s sarcoma-associated herpesvirus (KSHV) and is tested in Electron Microscopy, ELISA, Immunocytochemistry, Inhibition Assay, and Western Blotting.
Physical form
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Storage and Stability
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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