The T7-Tag® Antibody is a mouse monoclonal antibody (IgG2b) directed against the 11 amino acid gene 10 leader peptide expressed by many of the pET translation vectors as well as PEPscreen® and pRSET vectors. Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage (e.g., the T7Select® Vectors). For increased versatility and sensitivity, this antibody is also available labeled with biotin and as a direct conjugate with alkaline phosphatase or horseradish peroxidase.
Specificity
T7•Tag peptide MetAlaSerMetThrGlyGlyGlnGlnMetGly; N-terminal, C-terminal or internal
Cross-reactivity: Negligible with bacterial, insect or mammalian cell lysates
Sensitivity: 1–10 ng (Western blot developed with chromogenic substrates) < 1 ng (AP or HRP conjugate developed with chemiluminescent substrates, or Biotin conjugate developed with Streptavidin AP and chromogenic substrates). Also suitable for immunoprecipitation and immunohistochemistry (1–3)
Working dilution- 1:5,000–1:10,000
Packaging
Plastic ampoule
Warning
Multiple Toxicity Values, refer to MSDS
Physical form
Stabilized solution of antibody in 50% glycerol; includes Positive Control Extract containing 31.1 kDa target protein
Other Notes
Krek, W., et al. 1994. Cell 78,, 161.
Roberts, S.G.E. and Green, M. (1994) Nature 371, 717–720.
Joneson, T., McDonough, M., Bar-Sag, D., and Van Aelst, L. (1996) Science 274, 1374–1376.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
PEPscreen is a registered trademark of Sigma-Aldrich Co. LLC
T7-Tag is a registered trademark of Merck KGaA, Darmstadt, Germany
T7SELECT is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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