Skip to Content
Merck
All Photos(3)

Key Documents

View All Documentation

MAK009

Sigma-Aldrich

Amylase Activity Assay Kit

sufficient for 100 colorimetric tests

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

endocrinological disorders, diabetes; gastrointestinal diseases

storage temp.

−20°C

Gene Information

human ... AMY1A(278)

General description

The new Amylase Assay Kit, MAK478, is now available! Amylase enzymes are glycoside hydrolases, which cleave glucan linkages in polysaccharides such as starch or glycogen to release polysaccharide molecules. α-Amylase is found in animals, plants, and many fungi.[1] In mammals, α-amylase is the predominant form of amylase and is one of the major enzymes of dietary digestion. Both the activity and the stability of α-amylase is dependent on calcium ions. In humans, α-amylase levels are highest in pancreatic and salivary secretions.[2] Increased blood and urine levels of amylase occur due to pancreatic disorders and salivary trauma. Increased salivary amylase activity is also a marker for increased sympathetic nervous system activity.[3][4]

Application

Amylase Activity Assay Kit has been used to determine the activity of amylase.[5][6][7]

Suitability

Suitable for the quantification of amylase activity in a variety of biological samples.

Principle

The Amylase Activity Assay kit quantifies amylase activity in a variety of biological samples. The assay is quick, convenient, and sensitive. In this kit, amylase activity is determined using a coupled enzymatic assay, which results in a colorimetric (405 nm) product, proportional to the amount of substrate, ethylidene-pNP-G7, cleaved by the amylase. One unit is the amount of amylase that cleaves ethylidene-pNP-G7 to generate 1.0 μmole of p-nitrophenol per minute at 25 °C.

replaced by

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
8L03K07110
8J27K07110
8E24K07110
7M09K07110
8C09K07110

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Philip van der Zee et al.
Purinergic signalling (2018-05-13)
Recent research suggested an important role for pulmonary extracellular adenosine triphosphate (ATP) in the development of ventilation-induced lung injury. This injury is induced by mechanical deformation of alveolar epithelial cells, which in turn release ATP to the extracellular space. Measuring
Usefulness of salivary alpha amylase as a biomarker of chronic stress and stress related oral mucosal changes?a pilot study.
Vineetha R, et al.
Journal of Clinical and Experimental Dentistry, 6(2), e132-e132 (2014)
Natalie Chiu et al.
Food hydrocolloids, 69, 450-458 (2017-08-05)
Sodium (salt) was encapsulated within the inner water phase of w1/o/w2 food emulsions externally stabilised by starch particles with the ultimate aim of enhancing saltiness perception. The physical properties of the starch particles were modified by octenyl succinic anhydride (OSA)
X Chen et al.
Poultry science, 95(4), 834-841 (2016-01-08)
A 14-d study was conducted to determine the impact of dietary crude protein concentration on performance, serum biochemistry, and nutrient digestive functions in Pekin ducklings during aflatoxicosis. A total of 144 male Pekin ducklings were randomly allotted to 4 dietary
Galactose protects against cell damage in mouse models of acute pancreatitis.
Peng S, et al.
The Journal of Clinical Investigation (2018)
View All Related Papers

Questions

1–4 of 4 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. Hi There, is this ELISA Kit? many thanks, Ghaida.

    1 answer

    1. This kit is not an ELISA assay, as there are no antibodies involved. The principle of this kit is that alpha-Amylase cleaves the substrate ethylidene-pNP-G7 to produce smaller fragments that are eventually modified by alpha-glucosidase. P-nitrophenol is then released, which leads to the color development that is monitored by absorbance at 405 nm spectrophotometrically.

      Helpful?

  4. I want to know the name of each component of Amylase Activity Assay Kit

    1 answer

    1. The components of this kit are listed on the Product Datasheet which can be located in the 'DOCUMENTATION' section under 'More Documents':
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/377/793/mak009bul.pdf

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service