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MicroRNA-183 increases osteoclastogenesis by repressing heme oxygenase-1.

MicroRNA-183 increases osteoclastogenesis by repressing heme oxygenase-1.

Bone (2015-07-15)

Ke Ke, Ok-Joo Sul, Monisha Rajasekaran, Hye-Seon Choi

ABSTRACT

Emerging evidence suggests that microRNAs (miRs) influence skeletal structure by modulating osteoclastogenesis and bone resorption. We have demonstrated previously that the up-regulation of heme oxygenase-1 (HO-1) attenuated osteoclastogenesis in bone marrow-derived macrophages (BMMs). RANKL-induced osteoclastogenesis elevates microRNA-183 (miR-183) in BMM. We show here that HO-1 is a target gene of miR-183 and that this miRNA binds to the 3'-UTR of HO-1. We find that a synthetic inhibitor that binds to miR-183 decreases osteoclast (OC) differentiation and increases the expression of HO-1, while a mimic of endogenous mature miR-183 has the opposite effect. Moreover, the HO-1 inducers, resveratrol and piceatannol decrease the expression of miR-183, resulting in attenuated osteoclastogenesis. Our findings reveal how miR-183 affects OC formation.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Piceatannol, powder

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MISSION^® esiRNA, targeting human HMOX1

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MISSION^® esiRNA, targeting mouse Hmox1

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Dimethyl sulfoxide, BioUltra, for molecular biology, ≥99.5% (GC)

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Dimethyl sulfoxide, anhydrous, ≥99.9%

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Resveratrol, ≥99% (HPLC)

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Hematoxylin, certified by the Biological Stain Commission

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Hematoxylin

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Dimethyl sulfoxide, PCR Reagent

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Ammonium hydroxide solution, ACS reagent, 28.0-30.0% NH₃ basis

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Dimethyl sulfoxide, ACS reagent, ≥99.9%

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Dimethyl sulfoxide, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma

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Dimethyl sulfoxide, meets EP testing specifications, meets USP testing specifications

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Dimethyl sulfoxide, for molecular biology

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Dimethyl sulfoxide, Hybri-Max^™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

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Ammonium hydroxide solution, puriss., 30-33% NH₃ in H₂O

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Ammonium hydroxide solution, puriss., meets analytical specification of Ph. Eur., 25-30% NH₃ basis

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Dimethyl sulfoxide, ReagentPlus^®, ≥99.5%

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Ammonium hydroxide solution, puriss. p.a., reag. ISO, reag. Ph. Eur., ~25% NH₃ basis

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Ammonium hydroxide solution, puriss. p.a. plus, ≥25% NH₃ in H₂O

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Dimethyl sulfoxide, puriss. p.a., dried, ≤0.02% water

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Ammonium hydroxide solution, 28% NH₃ in H₂O, ≥99.99% trace metals basis

Sigma-Aldrich

Dimethyl sulfoxide, puriss. p.a., ACS reagent, ≥99.9% (GC)