Silver, zinc oxide and titanium dioxide nanoparticle ecotoxicity to bioluminescent Pseudomonas putida in laboratory medium and artificial wastewater.

Silver, zinc oxide and titanium dioxide nanoparticle ecotoxicity to bioluminescent Pseudomonas putida in laboratory medium and artificial wastewater.

Environmental pollution (Barking, Essex : 1987) (2014-09-28)

Florian Mallevre, Teresa F Fernandes, Thomas J Aspray

ABSTRACT

Bacteria based ecotoxicology assessment of manufactured nanoparticles is largely restricted to Escherichia coli bioreporters in laboratory media. Here, toxicity effects of model OECD nanoparticles (Ag NM-300K, ZnO NM-110 and TiO2 NM-104) were assessed using the switch-off luminescent Pseudomonas putida BS566::luxCDABE bioreporter in Luria Bertani (LB) medium and artificial wastewater (AW). IC50 values ∼4 mg L(-1), 100 mg L(-1) and >200 mg L(-1) at 1 h were observed in LB for Ag NM-300K, ZnO NM-110 and TiO2 NM-104, respectively. Similar results were obtained in AW for Ag NM-300K (IC50∼5 mg L(-1)) and TiO2 NM-104 (IC50>200 mg L(-1)) whereas ZnO NM-110 was significantly higher (IC50>200 mg L(-1)). Lower ZnO NM-110 toxicity in AW compared to LB was associated with differences in agglomeration status and dissolution rate. This work demonstrates the importance of nanoecotoxicological studies in environmentally relevant matrices.

MATERIALS

Product Number

Brand

Product Description

GF24976525

Titanium, rod, 200mm, diameter 25mm, annealed, 99.6+%

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Titanium, tube, 200mm, outside diameter 10.3mm, inside diameter 8.7mm, wall thickness 0.8mm, annealed, 99.6+%

GF41437830

Titanium, tube, 100mm, outside diameter 1.05mm, inside diameter 0.75mm, wall thickness 0.15mm, annealed, 99.6+%

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Titanium, rod, 500mm, diameter 1.5mm, annealed, 99.6+%

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Titanium, rod, 25mm, diameter 50mm, 99.99+%

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Titanium, tube, 200mm, outside diameter 6.35mm, inside diameter 5.53mm, wall thickness 0.41mm, annealed, 99.6+%

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Titanium, tube, 100mm, outside diameter 6.1mm, inside diameter 5.1mm, wall thickness 0.5mm, annealed, 99.6+%

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Titanium, tube, 1000mm, outside diameter 4.25mm, inside diameter 3.75mm, wall thickness 0.25mm, annealed, 99.6+%

GF25290581

Titanium, tube, 1000mm, outside diameter 25.4mm, inside diameter 23.62mm, wall thickness 0.89mm, annealed, 99.6+%

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Titanium, rod, 500mm, diameter 5mm, annealed, 99.6+%

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Titanium, rod, 200mm, diameter 16mm, annealed, 99.6+%

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Titanium, rod, 100mm, diameter 16mm, as drawn, 99.99+%

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Titanium, tube, 100mm, outside diameter 6.35mm, inside diameter 5.53mm, wall thickness 0.41mm, annealed, 99.6+%

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Titanium, tube, 200mm, outside diameter 25.4mm, inside diameter 23.62mm, wall thickness 0.89mm, annealed, 99.6+%

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Titanium, tube, 100mm, outside diameter 2.03mm, inside diameter 1.55mm, wall thickness 0.24mm, annealed, 99.6+%

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Titanium, tube, 1000mm, outside diameter 1.6mm, inside diameter 1.2mm, wall thickness 0.2mm, hard, 99.6+%

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Titanium, rod, 50mm, diameter 20mm, as drawn, 99.99+%

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Titanium, rod, 200mm, diameter 9.5mm, annealed, 99.99+%

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Titanium, rod, 490mm, diameter 2mm, annealed, 99.6+%

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Titanium, tube, 1220mm, outside diameter 2.03mm, inside diameter 1.55mm, wall thickness 0.24mm, annealed, 99.6+%