Effect of nicotinamide and its two derivatives on the generation of reactive oxygen species in human monocytes cooperating with platelets.

The study was designed to demonstrate the relationship between the activity of human normal monocytes and blood platelets, to determine the metabolic activity of normal monocytes and monocytes cooperating with blood platelets in respect of their generation of reactive oxygen species (ROS) and to study the response of cooperating cells to nicotinamide (NA), 1-methylnicotinamide (MNA+) and 1-methyl-N'-hydroxymethylnicotinamide (MNAF+). The ability of those potential antiinflammatory compounds to inhibit oxygen respiratory burst was also assessed. Measurements were carried out by luminol chemiluminometry. The results of the measurements were compared to the data acquired for aspirin (ASA). The results obtained showed that MNAF inhibited oxygen burst in monocytes cooperating with platelets, whereas the two other compounds, NA and MNA, did not cause inhibition of oxygen burst in vitro at a statistically significant level.