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  • Different sample treatment approaches for the analysis of T-2 and HT-2 toxins from oats-based media.

Different sample treatment approaches for the analysis of T-2 and HT-2 toxins from oats-based media.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (2010-06-29)
Angel Medina, Francisco M Valle-Algarra, Misericordia Jiménez, Naresh Magan
ABSTRACT

A LC-DAD method is proposed for the determination of the T-2 and HT-2 toxins in cultures of Fusarium langsethiae in oat-based and other in vitro media. Test media consisted of freshly prepared milled oats to which T-2 and HT-2 toxin stock solutions were added. Different mixtures of extraction solvent (acetonitrile:water and methanol:water), extraction times (30', 60' or 90') and drying methods were investigated. Results showed that extraction with methanol:water (80:20, v/v) for 90 min, drying with N(2) and subsequent analysis by LC-DAD was the fastest and most user friendly method for detecting HT-2 and T-2 toxins production by F. langsethiae strains grown on oat-based media at levels of 0.459 and 0.508 mg of toxin/kg of agar, respectively. The proposed method was used to investigate toxin production of 6 F. langsethiae strains from northern Europe and provided clear chromatograms with no interfering peaks in media with and without glycerol as water activity modifier.

MATERIALS
Product Number
Brand
Product Description

Supelco
HT-2 Toxin solution, 100 μg/mL in acetonitrile, analytical standard
Sigma-Aldrich
HT-2 Toxin, ≥98% (HPLC)