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  • Fluorogenic selective and differential medium for isolation of Enterobacter sakazakii.

Fluorogenic selective and differential medium for isolation of Enterobacter sakazakii.

Applied and environmental microbiology (2004-09-04)
Se-Wook Oh, Dong-Hyun Kang
ABSTRACT

4-Methylumbelliferyl-alpha-D-glucoside, the fluorogenic substrate of alpha-glucosidase, was used as a selective marker to develop a differential medium for Enterobacter sakazakii. This bacterium showed strong fluorogenic characteristics clearly distinguishable from other microorganisms. On the basis of reducing background noise, an optimum basal medium and nitrogen source were selected. Incubation conditions were optimized.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
4-Methylumbelliferyl α-D-glucopyranoside, ≥98% (TLC)