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  • Distribution of planar cell polarity proteins in the developing avian retina.

Distribution of planar cell polarity proteins in the developing avian retina.

Experimental eye research (2021-06-25)
Guadalupe Álvarez-Hernán, Sergio Garrido-Jiménez, Ángel Carlos Román, José María Carvajal-González, Javier Francisco-Morcillo
ABSTRACT

Planar cell polarity (PCP) is evolutionary conserved and play a critical role in proper tissue development and function. During central nervous system development, PCP proteins exhibit specific patterns of distribution and are indispensable for axonal growth, dendritogenesis, neuronal migration, and neuronal differentiation. The retina constitutes an excellent model in which to study molecular mechanisms involved in neural development. The analysis of the spatiotemporal expression of PCP proteins in this model constitutes an useful histological approach in order to identify possible roles of these proteins in retinogenesis. Immunohistochemical techniques revealed that Frz6, Celsr1, Vangl1, Pk1, Pk3, and Fat1 were present in emerging axons from recently differentiated ganglion cells in the chicken retina. Except for Vangl1, they were also asymmetrically distributed in differentiated amacrine cells. Pk1 and Pk3 were restricted in the outer nuclear layer to the outer segment of photoreceptors. Vangl1 was also located in the cell somata of Müller glia. Given these findings together, the distribution of PCP proteins in the developing chicken retina suggest essential roles in axonal guidance during early retinogenesis and a possible involvement in the establishment of cell asymmetry and maintenance of retinal cell phenotypes.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-VANGL1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-Prox1 antibody , Mouse monoclonal, clone 4G10, purified from hybridoma cell culture
Sigma-Aldrich
Anti-Glutamine Synthetase Antibody, clone GS-6, clone GS-6, Chemicon®, from mouse
Sigma-Aldrich
Anti-Parvalbumin Antibody, ascites fluid, clone PARV-19, Chemicon®
Sigma-Aldrich
Anti-FAT1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-PRICKLE3 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody