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  • Structure and associated DNA-helicase activity of a general transcription initiation factor that binds to RNA polymerase II.

Structure and associated DNA-helicase activity of a general transcription initiation factor that binds to RNA polymerase II.

Nature (1989-10-05)
M Sopta, Z F Burton, J Greenblatt
PMID2477704
ABSTRACT

RAP30/74 is a heteromeric general transcription initiation factor which binds to RNA polymerase II. Here we report that preparations of RAP30/74 contain an ATP-dependent DNA helicase whose probable function is to melt the DNA at transcriptional start sites. The sequence of the RAP30 subunit of RAP30/74 indicates that RAP30 may be distantly related to bacterial sigma factors.

MATERIALS
Product Number
Brand
Product Description
SRP2007
Sigma-Aldrich
TFIIF (RAP74 subunit) human, recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
SRP2008
Sigma-Aldrich
TFIIF (RAP30 subunit) human, recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
SRP2009
Sigma-Aldrich
TFIIF (RAP30+Rap74) human, recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
SRP2114
Sigma-Aldrich
TFIIF, Rap30 subunit, GST tagged human, recombinant, expressed in E. coli, ≥80% (SDS-PAGE)