ZRB2171
Anti-SAFB Antibody, clone 2N15-L2 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
HSP27 ERE-TATA-binding protein, HSP27 estrogen response element-TATA box-binding protein, SAF-B, SAF-B1, Scaffold attachment factor B1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
2N15-L2, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
purified by
using Protein A
species reactivity
human, mouse
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
epitope sequence
Internal
Protein ID accession no.
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 2N15-L2 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Scaffold attachment factor B1 (SAFB). It targets an epitope within 24 amino acids from the internal region.
Immunogen
KLH-conjugated linear peptide corresponding to 24 amino acids from the internal region of human Scaffold attachment factor B1 (SAFB).
Application
Quality Control Testing
Evaluated by Western Blotting in NIH3T3 cell nuclear extract.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SAFB in NIH3T3 cell nuclear extract.
Tested Applications
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected SAFB in A431 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SAFB in Human kidney tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in NIH3T3 cell nuclear extract.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SAFB in NIH3T3 cell nuclear extract.
Tested Applications
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected SAFB in A431 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SAFB in Human kidney tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Scaffold attachment factor B1 (UniProt: Q15424; also known as SAF-B, SAF-B1, HSP27 estrogen response Element™-TATA box-binding protein, HSP27 ERE-TATA-binding protein) is encoded by the SAFB (also known as HAP, HET, SAFB1) gene (Gene ID: 6294) in human. The scaffold attachment factor protein family consists of SAFB1, SAFB2 and a SAF-like transcription modulator (SLTM). SBAF1 is an evolutionarily conserved, monomeric or homodimeric, RNA binding protein that is involved in several processes, including transcription, chromatin structure, stress response, DNA repair, and apoptosis. It can also form heterodimers with SAFB2. It is ubiquitously expressed with highest levels observed in the central nervous system. Its expression is also observed in breast cancer cell lines. In neurons, its expression is regulated by stress, suggesting its role in coordinating the expression of neuroprotective proteins. It binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a transcriptosomal complex for coupling transcription and RNA processing. SAFB1 also interacts with proteins involved in splicing and other aspects of RNA-processing. Four isoforms of SABF1 have been described that are produced by alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity™, reproducibility, and stability over conventional monoclonals. (Ref.: Hong, EA., et al. (2012). Biochem. Soc. Trans. 40(4); 826-830; Rivers, C., et al. (2015). BMC Biology. 13; Article 111; Nayler, O., et al. (1998). Nucleic Acids Res. 26(15); 3542-3549).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
Affinity is a trademark of Mine Safety Appliances Co.
Element is a trademark of Corning, Inc.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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