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  • Callus cultures of Annona squamosa for the production of annonaceous acetogenins.

Callus cultures of Annona squamosa for the production of annonaceous acetogenins.

Journal of Asian natural products research (2002-07-18)
Li-Da Wang, De-You Qiu, Ji-Yun Chen, Yi-Fan Han, Jun-Hua Zheng, De-An Guo
ABSTRACT

Callus cultures of Annona squamosa were induced using different explants including petals, seed contents (megagametophyte and embryo) and fruits (mesocarp). Growth of the calli induced from the explants was found to be influenced by the type, concentration and ratio of auxin vs. cytokinin. The content of squamocin (67.8 microg g(-1) dry weight) in calli cultured on Gamborg B-5 medium containing 5.0 mg l(-1) naphthalene acetic acid and 4.0 mg l(-1) zeatin was nearly seven times higher than that in intact fruits.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Gamborg′s Vitamin Solution, 1000 ×, liquid, suitable for plant cell culture
Sigma-Aldrich
Gamborg′s B-5 Basal Medium with Minimal Organics, fine powder, suitable for plant cell culture
Sigma-Aldrich
Gamborg′s B-5 Basal Salt Mixture, fine powder, suitable for plant cell culture