SAB4200559

Anti-VAChT (C-terminal) antibody produced in rabbit

IgG fraction of antiserum

• Synonym(s): Anti-SLC18A3, Anti-Solute carrier family 18 member 3, Anti-VAT, Anti-vesicular acetylcholine transporter
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: rat, mouse, human
• technique(s): indirect immunofluorescence: 1:1,000-1:2,000 using PC12 cells treated with rat NGF.; western blot: 1:500-1:800 using extracts of mouse brain (S1 fraction).
• UniProt accession no.: Q16572
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SLC18A3(6572) ; mouse ... slc18a3(20508) ; rat ... slc18a3(60422)

Description

General description

Vesicular Acetylcholine Transporter (VAChT, SLC18A3) belongs to the solute carrier family 18 (SLC18) family of proton/neurotransmitter antiporters and shares a high degree of homology to vesicular monoamine transporters (VMATs). It is a 12-transmembrane domain protein. VAChT is expressed in all major cholinergic neurons in the central and peripheral nervous system and is mainly localized in small synaptic vesicles in cholinergic nerve terminals.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminus of human vesicular acetylcholine transporter (VAChT), conjugated to KLH. The corresponding sequence is highly conserved in rat and mouse VAChT (82% identity).

Application

Anti-VAChT (C-terminal) antibody produced in rabbit has been used in:

• immunoblotting
• immunofluorescence staining
• immunocytochemistry
• double immunolabeling

Biochem/physiol Actions

Vesicular Acetylcholine Transporter (VAChT), is required in cholinergic neurons for the selective transport of acetylcholine (ACh) into synaptic vesicles. It depends on the activity of a vacuolar-type H⁺ -ATPase to accumulate ACh in synaptic vesicles. Knockdown of VAChT in the hippocampus has been shown to cause behavior changes, learning deficits, specific deficits in spatial memory and long-term potentiation (LTP). VAChT provides a specific marker for cholinergic neurons, for the study of cholinergic transmission in experimental models of Alzheimer′s disease and other nervous system disorders. Cloning of the VAChT gene has revealed a single genetic locus encoding both VAChT and ChAT, providing a unique genomic arrangement suggesting that these genes may be co-regulated.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable