Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Gene (2014-02-04)

Gonensin O Bozdag, Alaattin Kaya, Ahmet Koc, Gundula A Noll, Dirk Prüfer, Hüseyin Caglar Karakaya

ABSTRACT

Nickel is an essential micronutrient due to its involvement in many enzymatic reactions as a cofactor. However, excess of this element is toxic to biological systems. Here, we constructed a cDNA library from Beta maritima and screened it in the yeast system to identify genes that confer resistance to toxic levels of nickel. A cDNA clone (NIC6), which encodes for a putative membrane protein with unknown function, was found to help yeast cells to tolerate toxic levels of nickel. A GFP fused form of Nic6 protein was localized to multivesicular structures in tobacco epidermal cells. Thus, our results suggest a possible role of Nic6 in nickel and intracellular ion homeostasis.

MATERIALS

Product Number

Brand

Product Description

577995

Sigma-Aldrich

Nickel, nanopowder, <100 nm avg. part. size, ≥99% trace metals basis

266981

Sigma-Aldrich

Nickel, powder, <50 μm, 99.7% trace metals basis

357588

Sigma-Aldrich

Nickel, foil, thickness 0.1 mm, 99.98% trace metals basis

357626

Sigma-Aldrich

Nickel, wire, diam. 0.5 mm, ≥99.9% trace metals basis

267074

Sigma-Aldrich

Nickel, rod, diam. 6.35 mm, ≥99.99% trace metals basis

357553

Sigma-Aldrich

Nickel, foil, thickness 0.5 mm, 99.98% trace metals basis

GF73640505

Nickel, foil, 15mm disks, thickness 0.075mm, annealed, 99%

GF75780478

Nickel, foil, 15mm disks, thickness 0.025mm, as rolled, 100%

GF93376941

Nickel, tube, 100mm, outside diameter 0.75mm, inside diameter 0.59mm, wall thickness 0.08mm, hard, 99.5%

GF86704005

Nickel, foil, 10mm disks, thickness 0.30mm, as rolled, 99.98%

GF44029425

Nickel, foil, 1m coil, thickness 0.15mm, annealed, 99%

GF39742577

Nickel, rod, 200mm, diameter 20.0mm, 99%

GF63510308

Nickel, foil, 10mm disks, thickness 0.125mm, as rolled, 100%

GF10605967

Nickel, foil, 1m coil, thickness 0.3mm, coil width 1mm, as rolled, 99%

GF71201168

Nickel, sphere, 100pcs, diameter 0.76mm, precision sphere grade 200, 99%

GF15634054

Nickel, rod, 500mm, diameter 9.5mm, 99%

GF44855073

Nickel, foil, 15mm disks, thickness 0.25mm, as rolled, 100%

GF41132708

Nickel, tube, 100mm, outside diameter 3.25mm, inside diameter 3.05mm, wall thickness 0.1mm, hard, 99.5%

GF98733314

Nickel, tube, 100mm, outside diameter 0.8mm, inside diameter 0.5mm, wall thickness 0.15mm, as drawn, 99.5%

GF85929069

Nickel, tube, 1000mm, outside diameter 3.0mm, inside diameter 2.4mm, wall thickness 0.3mm, hard, 99.5%

GF98441679

Nickel, foil, 1m coil, thickness 0.003mm, 99.95%

GF42115115

Nickel, foil, 15mm disks, thickness 0.5mm, as rolled, 99.99+%

GF03364499

Nickel, rod, 50mm, diameter 5.0mm, 99.99+%

GF54839188

Nickel, tube, 100mm, outside diameter 2.5mm, inside diameter 2mm, wall thickness 0.25mm, hard, 99.5%

GF84917582

Nickel, tube, 200mm, outside diameter 0.65mm, inside diameter 0.384mm, wall thickness 0.133mm, as drawn, 99.5%

GF43735800

Nickel, tube, 100mm, outside diameter 0.35mm, inside diameter 0.3mm, wall thickness 0.025mm, hard, 99.5%

GF27334425

Nickel, foil, 1m coil, thickness 0.125mm, annealed, 99.98%

GF23820128

Nickel, foil, 10mm disks, thickness 0.3mm, as rolled, 100%

GF62945691

Nickel, foil, 15mm disks, thickness 0.025mm, annealed, 99%

GF38194386

Nickel, rod, 50mm, diameter 10.0mm, 99.99+%