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  • Complement activation by 3-mercapto-1,2-propanediol immobilized on gold surfaces.

Complement activation by 3-mercapto-1,2-propanediol immobilized on gold surfaces.

Biomaterials (1996-05-01)
P Tengvall, A Askendal, I Lundström
ABSTRACT

Thiol-modified surfaces are chemically well defined and suited for surface biological model experiments and biomaterials research. 3-Mercapto-1,2-propanediol (mercaptoglycerol, MG), immobilized on gold, spontaneously binds immunoglobulins from human serum and activates the complement system. The surface-bound complement factors were detected by ellipsometry-antibody techniques. The overall complement activation was subsequently corroborated independently with enzyme immunosorbent assay (EIA) and sheep and chicken erythrocyte haemolytic complement techniques. EIA experiments indicated elevated levels of C4d, but no significant increase of factor Bb was evident in the test serum from the MG system. The haemolytic assays show that MG surfaces consume complement factors from both pathways. Ellipsometry revealed that immunoglobulin G (IgG) and complement factor 1q (C1q) are transiently antibody detectable on MG after exposure to whole serum by the use of antibody techniques. Complement factor 3 (C3), C2, C4 and properdin could be detected on the surface, but not factors H and B. The total adsorbed mass and particularly C3 antibody deposition were suppressed by using EGTA-Mg2+ serum. The results suggest that MG surfaces initially activate complement via the classical pathway. Other IgG binding surfaces also appear to behave in a similar manner.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
1-Thioglycerol, ≥99.0% (GC)
Sigma-Aldrich
1-Thioglycerol, ≥97%
Sigma-Aldrich
1-Thioglycerol, BioXtra, ≥97%
Sigma-Aldrich
1-Thioglycerol, liquid, BioReagent, suitable for cell culture, ≥97% (titration)