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  • Spatiotemporal distribution of chondroitin sulfate proteoglycans after optic nerve injury in rodents.

Spatiotemporal distribution of chondroitin sulfate proteoglycans after optic nerve injury in rodents.

Experimental eye research (2019-11-11)
Craig S Pearson, Andrea G Solano, Sharada M Tilve, Caitlin P Mencio, Keith R Martin, Herbert M Geller
ABSTRACT

The accumulation of chondroitin sulfate proteoglycans (CSPGs) in the glial scar following acute damage to the central nervous system (CNS) limits the regeneration of injured axons. Given the rich diversity of CSPG core proteins and patterns of GAG sulfation, identifying the composition of these CSPGs is essential for understanding their roles in injury and repair. Differential expression of core proteins and sulfation patterns have been characterized in the brain and spinal cord of mice and rats, but a comprehensive study of these changes following optic nerve injury has not yet been performed. Here, we show that the composition of CSPGs in the optic nerve and retina following optic nerve crush (ONC) in mice and rats exhibits an increase in aggrecan, brevican, phosphacan, neurocan and versican, similar to changes following spinal cord injury. We also observe an increase in inhibitory 4-sulfated (4S) GAG chains, which suggests that the persistence of CSPGs in the glial scar opposes the growth of CNS axons, thereby contributing to the failure of regeneration and recovery of function.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Monoclonal Anti-Chondroitin Sulfate antibody produced in mouse, clone CS-56, ascites fluid
Sigma-Aldrich
Anti-Versican Antibody, a.a. 535-598 of mouse versican, Chemicon®, from rabbit
Millipore
MCE Membrane Filter, 0.8 μm Pore Size, MF-Millipore, filter diam. 13 mm, hydrophilic, black, gridded
Sigma-Aldrich
Anti-Aggrecan Antibody, Chemicon®, from rabbit
Sigma-Aldrich
CTB, ≥98% (HPLC)