SRP6445

TIMP1 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonym(s): CLGI, EPA, EPO, FLJ90373, HCI, TIMP, TIMP metallopeptidase inhibitor 1, TIMP-1
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in HEK 293 cells
• tag: 6-His tagged (C-terminus)
• Assay: ≥95% (SDS-PAGE)
• form: lyophilized
• mol wt: calculated mol wt 24 kDa; observed mol wt 28-32 kDa by SDS-PAGE (DTT-reduced.)
• packaging: pkg of 10 μg
• impurities: <1 EU/μg endotoxin (LAL test)
• UniProt accession no.: P01033
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... TIMP1(7076)

Description

General description

TIMP1 (tissue inhibitor of metalloproteinase 1) is a glycoprotein and is a member of TIMP family of endogenous MMP (matrix metalloproteinase) inhibitors. Humans contain four TIMPs. All TIMPs contain an N-terminal of 125 residues, a 65-residue C-terminal, and both these domains contain three disulfide bonds. The N-terminal domain inhibits MMPs by folding into a separate unit. TIMP1 is encoded by the gene mapped to human chromosome Xp11.23.

Biochem/physiol Actions

TIMP1 functions as a poor inhibitor of MT1 (membrane type 1)-MMP, MT3-MMP, MT5-MMP and MMP-19. It inhibits ADAM10 (a disintegrin and metalloproteinase domain-containing protein 10) protein. In human umbilical vein endothelial cells (HUVECs), the down-regulation of TIMP1 and up-regulation of MMP-3 results in aberrant endothelium-dependent vasodilation, EC (endothelial cell) death, and endothelial interruption in a FOXO3 (forkhead box O3)-mediated manner. In patients with CAD (coronary artery disease) and acute coronary syndrome (ACS), the urine levels of this protein are elevated. This protein interacts with Bcl-2 (B cell lymphoma) protein, and induces apoptosis in lung adenocarcinoma cells. The plasma levels of this protein are increased in patients with obesity and obesity-related disorders, such as steatosis, where it participates in pathogenesis of diet-induced hepatic steatosis and glucose intolerance.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable